Lipidomic analysis of diatoms cultivated with silica nanoparticles

0531963 - MBÚ 2021 RIV GB eng J - Journal Article
Řezanka, Tomáš - Řezanka, M. - Mezricky, D. - Vítová, Milada
Lipidomic analysis of diatoms cultivated with silica nanoparticles.
Phytochemistry. Roč. 177, SEP 2020 (2020), č. článku 112452. ISSN 0031-9422. E-ISSN 1873-3700
EU Projects: European Commission(CZ) ATCZ172 - Interreg V-A Austria – Czech Republic
Institutional support: RVO:61388971
Keywords : Diadesmidaceae * Navicula atomus * Naviculaceae
OECD category: Plant sciences, botany
Impact factor: 4.072, year: 2020
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0031942220303642

Polar lipids from the diatoms Diadesmis gallica and Navicula atomus were separated and their structures were determined using high resolution tandem MS HILIC-LC/ESI. This method allowed us to identify 34 classes of lipids, each containing dozens of molecular species, including regioisomers. The largest differences were found in two sulfur-containing lipids, sulfoquinovosyldiacylglycerol and phosphatidylsulfocholine caused probably by the remodeling of lipid species. These diatoms have been found to use several mechanisms to resolve growth in extreme environments, i.e. silica starvation. The presence of insoluble nano-SiO2 leads to the replacement of cellular phospholipids with sulfolipids. Regioisomer ratios also vary depending on the concentration of nano-SiO2 in the culture medium, i.e. the biosynthesis of polar lipids via the prokaryotic (plastidial) and/or eukaryotic (explastidial) pathways. Complex analyses of polar lipids using high resolution HILIC-LC/ESI-tandem, as used for diatoms, can also be used for other photosynthetic microorganisms.
Permanent Link: http://hdl.handle.net/11104/0310587