In Vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes

0531959 - ÚEB 2021 RIV CH eng J - Journal Article
Kashkan, Ivan - Timofeyenko, Ksenia - Kollárová, E. - Růžička, Kamil
In Vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes.
Plants. Roč. 9, č. 7 (2020), č. článku 868. E-ISSN 2223-7747
R&D Projects: GA MŠMT(CZ) EF16_019/0000738; GA MŠMT(CZ) LTC18073; GA MŠMT(CZ) LM2018129; GA MŠMT(CZ) EF16_013/0001775
Grant - others:OPPK(XE) CZ.2.16/3.1.00/21519
Institutional support: RVO:61389030
Keywords : dependent auxin gradients * network * growth * cytochrome-p450 * proteins * cells * alternative splicing * auxin * cytokinins * brassinosteroids * ahp6 * dwf4 * pin7 * D-amino acid oxidase * genetic screen * Arabidopsis
OECD category: Biochemical research methods
Impact factor: 3.935, year: 2020
Method of publishing: Open access
http://doi.org/10.3390/plants9070868

Rapid progress in plant molecular biology in recent years has uncovered the main players in hormonal pathways and characterized transcriptomic networks associated with hormonal response. However, the role of RNA processing, in particular alternative splicing (AS), remains largely unexplored. Here, using example genes involved in cytokinin signaling, brassinosteroid synthesis and auxin transport, we present a set of reporters devised to visualize their AS events in vivo. These reporters show a differential tissue-specific expression of certain transcripts and reveal that expression of some of the them can be changed by the application of the exogenous hormone. Finally, based on the characterized AS event of the PIN7 auxin efflux carrier, we designed a system that allows a rapid genetic screening for the factors upstream of this AS event. Our innovative toolset can be therefore highly useful for exploring novel regulatory nodes of hormonal pathways and potentially helpful for plant researchers focusing on developmental aspects of AS.
Permanent Link: http://hdl.handle.net/11104/0310583