Biphasic optimization approach for maximization of lipid production by the microalga Chlorella pyrenoidosa

0531845 - ÚVGZ 2021 RIV CZ eng J - Journal Article
Sukačová, Kateřina - Búzová, Diana - Červený, Jan
Biphasic optimization approach for maximization of lipid production by the microalga Chlorella pyrenoidosa.
Folia Microbiologica. Roč. 65, č. 5 (2020), s. 901-908. ISSN 0015-5632. E-ISSN 1874-9356
R&D Projects: GA MŠMT(CZ) LO1415; GA MŠMT(CZ) EF16_026/0008413
Institutional support: RVO:86652079
Keywords : nitrogen starvation * light-intensity * cultivation * vulgaris * growth * accumulation * biomass * ph * sp. * photobioreactor
OECD category: Microbiology
Impact factor: 2.099, year: 2020
Method of publishing: Limited access
https://link.springer.com/article/10.1007/s12223-020-00800-w

The aim of the study was to identify the optimum cultivation conditions for the microalgal growth and lipid production of the oleaginous microalga Chlorella pyrenoidosa Chick (IPPAS C2). Moreover, an appropriate NO3- concentration in the cultivation medium for maximized lipid accumulation was determined. The experimental design involved a biphasic cultivation strategy with an initial biomass accumulating phase under optimized light (400 mu mol/m(2) per s), temperature (25 degrees C), and elevated CO2 concentration in the air mixture (3%), followed by a mid-elevated CO2 concentration (0.5%) for lipid induction. The highest lipid yields of 172.47 +/- 18.1 and 179.65 +/- 25.4 mg/L per day were detected for NO3- concentrations of 100 and 150 mg/L. The optimization approach presented here led not only to the maximization of lipid yield but also to the development of a biphasic cultivation strategy easily applicable to the cultivation process without the necessity for algal cell harvesting between the first and second cultivation phases.
Permanent Link: http://hdl.handle.net/11104/0310484