Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

0531799 - ÚIACH 2022 RIV DE eng J - Journal Article
Dosedělová, V. - Itterheimová, P. - Kubáň, Petr
Analysis of bile acids in human biological samples by microcolumn separation techniques: A review.
Electrophoresis. Roč. 42, 1-2 (2021), s. 68-85. ISSN 0173-0835. E-ISSN 1522-2683
Institutional support: RVO:68081715
Keywords : tandem mass-spectrometry * quantitative-analysis * human plasma * human urine * clinical analysis * gc-ms * hplc-ms * Microcolumn separation
OECD category: Analytical chemistry
Impact factor: 3.595, year: 2021
Method of publishing: Limited access

Bile acids are a group of compounds essential for lipid digestion and absorption with a steroid skeleton and a carboxylate side chain usually conjugated to glycine or taurine. Bile acids are regulatory molecules for a number of metabolic processes and can be used as biomarkers of various disorders. Since the middle of the twentieth century, the detection of bile acids has evolved from simple qualitative analysis to accurate quantification in complicated mixtures. Advanced methods are required to characterize and quantify individual bile acids in these mixtures. This article overviews the literature from the last two decades (2000-2020) and focuses on bile acid analysis in various human biological samples. The methods for sample preparation, including the sample treatment of conventional (blood plasma, blood serum, and urine) and unconventional samples (bile, saliva, duodenal/gastric juice, feces, etc.) are shortly discussed. Eventually, the focus is on novel analytical approaches and methods for each particular biological sample, providing an overview of the microcolumn separation techniques, such as high-performance liquid chromatography, gas chromatography, and capillary electrophoresis, used in their analysis. This is followed by a discussion on selected clinical applications.
Permanent Link: http://hdl.handle.net/11104/0310414