PRP8A and PRP8B spliceosome subunits act coordinately to control pollen tube attraction in Arabidopsis thaliana

0531677 - ÚEB 2021 RIV GB eng J - Journal Article
Kulichová, Katarína - Kumar, Vinod - Steinbachová, Lenka - Klodová, Božena - Timofejeva, Ljudmilla - Juříček, Miloslav - Honys, David - Hafidh, Said
PRP8A and PRP8B spliceosome subunits act coordinately to control pollen tube attraction in Arabidopsis thaliana.
Development. Roč. 147, č. 11 (2020), č. článku dev186742. ISSN 0950-1991. E-ISSN 1477-9129
R&D Projects: GA ČR(CZ) GA17-23203S; GA ČR(CZ) GA18-02448S; GA ČR(CZ) GA19-01723S; GA MŠMT(CZ) LTAUSA18115; GA MŠMT(CZ) EF16_019/0000738; GA MŠMT(CZ) LM2018129
Grant - others:OPPK(XE) CZ.2.16/3.1.00/21519
Institutional support: RVO:61389030
Keywords : Cell-to-cell signaling * Cysteine-rich proteins * Pollen tube attraction and reception * Post-transcriptional regulation * Pre-mRNA PROCESSING factor 8 * Sexual reproduction * Splicing
OECD category: Plant sciences, botany
Impact factor: 6.868, year: 2020
Method of publishing: Open access
http://doi.org/10.1242/dev.186742

Precise guided pollen tube growth by the female gametophyte is a prerequisite for successful sexual reproduction in flowering plants. Cysteine-rich proteins (CRPs) secreted from the embryo sac are known pollen tube attractants perceived by pollen tube receptor-like kinases. How pre-mRNA splicing facilitates this cell-to-cell communication is not understood. Here, we report a novel function of Pre-mRNA PROCESSING factor 8 paralogs, PRP8A and PRP8B, as regulators of pollen tube attraction. Double mutant prp8a prp8b ovules cannot attract pollen tubes, and prp8a prp8b pollen tubes fail to sense the ovule's attraction signals. Only 3% of ovule-expressed genes were misregulated in prp8a prp8b Combination of RNA sequencing and the MYB98/LURE1.2-YFP reporter revealed that the expression of MYB98, LUREs and 49 other CRPs were downregulated, suggesting loss of synergid cell fate. Differential exon usage and intron retention analysis revealed autoregulation of PPR8A/PRP8B splicing. In vivo, PRP8A co-immunoprecipitates with splicing enhancer AtSF3A1, suggesting involvement of PRP8A in 3'-splice site selection. Our data hint that the PRP8A/PRP8B module exhibits spliceosome autoregulation to facilitate pollen tube attraction via transcriptional regulation of MYB98, CRPs and LURE pollen tube attractants.
Permanent Link: http://hdl.handle.net/11104/0310297