Response of cytokinins and nitrogen metabolism in the fronds of Pteris sp. Under arsenic stress

0531600 - ÚEB 2021 RIV US eng J - Journal Article
Pavlíková, D. - Zemanová, Veronika - Pavlík, Milan - Dobrev, Petre - Hnilička, F. - Motyka, Václav
Response of cytokinins and nitrogen metabolism in the fronds of Pteris sp. Under arsenic stress.
PLoS ONE. Roč. 15, č. 5 (2020), č. článku e0233055. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR(CZ) GA17-10591S; GA MŠMT(CZ) EF16_019/0000738
Institutional support: RVO:61389030
Keywords : FREE AMINO-ACIDS * MAIZE BETA-GLUCOSIDASE * TRANS-ZEATIN CONTENT
OECD category: Biochemical research methods
Impact factor: 3.240, year: 2020
Method of publishing: Open access
http://doi.org/10.1371/journal.pone.0233055

Given the close relationship between cytokinins (CKs), photosynthesis and nitrogen metabolism, this study assessed the effect of arsenic (As) contamination on these metabolic components in the As-hyperaccumulators Pteris cretica L. var. Albo-lineata (Pc-A) and var. Parkerii (Pc-P) as well as the As-non-hyperaccumulator Pteris straminea Mett. ex Baker (Ps). The ferns were cultivated in a pot experiment for 23 weeks in soil spiked with As at the levels 20 and 100 mg·kg-1. For the purpose of this study, the CKs were placed into five functionally different groups according to their structure and physiological roles: bioactive forms (bCKs, CK free bases), inactive or weakly active forms (dCKs, CK N-glucosides), transport forms (tCKs, CK ribosides), storage forms (sCKs, O-glucosides), and primary products of CK biosynthesis (ppbCKs, CK nucleotides). An important finding was higher CKs total content, accumulation of sCKs and reduction of dCKs in As-hyperaccumulators in contrast to non-hyperaccumulator ferns. A significant depletion of C resources was confirmed in ferns, especially Ps, which was determined by measuring the photosynthetic rate and chlorophyll fluorescence. A fluorescence decrease signified a reduction in the C/N ratio, inducing an increase of bioactive CKs forms in Pc-P and Ps. The impact of As on N utilization was significant in As-hyperaccumulators. The glutamic acid/glutamine ratio, an indicator of primary N assimilation, diminished in all ferns with increased As level in the soil. In conclusion, the results indicate a large phenotypic diversity of Pteris species to As and suggest that the CKs composition and the glutamic acid/glutamine ratio can be used as a tool to diagnose As stress in plants.
Permanent Link: http://hdl.handle.net/11104/0310232