New Insights Into the Metabolism and Role of CytokininN-Glucosides in Plants

0531598 - ÚEB 2021 RIV CH eng J - Journal Article
Hoyerová, Klára - Hošek, Petr
New Insights Into the Metabolism and Role of CytokininN-Glucosides in Plants.
Frontiers in Plant Science. Roč. 11, JUN 5 (2020), č. článku 741. ISSN 1664-462X. E-ISSN 1664-462X
R&D Projects: GA ČR(CZ) GA16-19557S; GA MŠMT(CZ) EF16_019/0000738
Institutional support: RVO:61389030
Keywords : arabidopsis-thaliana * biological-activity * biochemical-characterization * n-glucosylation * cis-zeatin * maize * expression * tobacco * glycosyltransferases * dehydrogenase * cytokininN-glucoside * isopentenyladenine N7-glucoside * isopentenyladenine N9-glucoside * zeatin N7-glucoside * zeatin N9-glucoside * cytokinin metabolism * cytokinin transport * ugt
OECD category: Biochemical research methods
Impact factor: 5.754, year: 2020
Method of publishing: Open access
http://doi.org/10.3389/fpls.2020.00741

Cytokinin (CK)N-glucosides are the most abundant group of CK metabolites in many species, however, their physiological rolein plantawas for a long time perceived as irreversible storage CK forms only. Recently, a comprehensive screen showed that only vascular plants form CKN-glucosides in contrast to mosses, algae, and fungi. The formation of CKN-glucosides as biologically inactive CK conjugates thus represents an evolutionarily young mechanism for deactivation of CK bases. Even though CKN-glucosides are not biologically active themselves due to their inability to activate the CK perception system, new data on CKN-glucoside metabolism show thattrans-zeatin (tZ) N7- and N9-glucosides are metabolizedin vivo, efficiently releasing free CK bases that are most probably responsible for the biological activities observed in a number of bioassays. Moreover, CKN-glucosides' subcellular localization as well as their abundance in xylem both point to their possible plasma membrane transport and indicate a role also as CK transport forms. Identification of the enzyme(s) responsible for the hydrolysis of tZ N7- and N9-glucosides, as well as the discovery of putative CKN-glucoside plasma membrane transporter, would unveil important parts of the overall picture of CK metabolic interconversions and their physiological importance.
Permanent Link: http://hdl.handle.net/11104/0310230