Factors Affecting the Function of the Mitochondrial Membrane Permeability Transition Pore and Their Role in Evaluation of Calcium Retention Capacity Values

0531512 - FGÚ 2021 RIV CZ eng J - Journal Article
Drahota, Zdeněk - Endlicher, R. - Kučera, O. - Rychtrmoc, D. - Červinková, Z.
Factors Affecting the Function of the Mitochondrial Membrane Permeability Transition Pore and Their Role in Evaluation of Calcium Retention Capacity Values.
Physiological Research. Roč. 69, č. 3 (2020), s. 491-499. ISSN 0862-8408. E-ISSN 1802-9973
Institutional support: RVO:67985823
Keywords : liver mitochondria * calcium retention capacity * mitochondrial permeability transition pore
OECD category: Biochemistry and molecular biology
Impact factor: 1.881, year: 2020
Method of publishing: Open access
http://www.biomed.cas.cz/physiolres/pdf/2020/69_491.pdf

Values of the calcium retention capacity (CRC) of rat liver mitochondria are highly dependent on the experimental conditions used. When increasing amounts of added calcium chloride are used (1.25-10 nmol), the values of the CRC increase 3-fold. When calcium is added in 75 s intervals, the CRC values increase by 30 % compared with 150 s interval additions. CRC values are not dependent on the calcium/protein ratio in the measured sample in our experimental design. We also show that a more detailed evaluation of the fluorescence curves can provide new information about mitochondrial permeability transition pore opening after calcium is added.
Permanent Link: http://hdl.handle.net/11104/0310155