Enhancement of Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels by Plant-Derived Gallotannins

0531321 - FGÚ 2021 RIV CH eng J - Journal Article
Douglas, T.E.L. - Keppler, J. K. - Vandrovcová, Marta - Plencner, Martin - Beranová, J. - Feuereisen, M. - Parakhonskiy, B. V. - Svenskaya, Y. - Atkin, V. - Ivanova, A. - Ricquier, P. - Balcaen, L. - Vanhaecke, F. - Schieber, A. - Bačáková, Lucie - Skirtach, A. G.
Enhancement of Biomimetic Enzymatic Mineralization of Gellan Gum Polysaccharide Hydrogels by Plant-Derived Gallotannins.
International Journal of Molecular Sciences. Roč. 21, č. 7 (2020), č. článku 2315. E-ISSN 1422-0067
R&D Projects: GA ČR(CZ) GA17-00885S
Institutional support: RVO:67985823
Keywords : mineralization * polyphenol * composite * protein-polyphenol interaction * gellan gum * enzyme
OECD category: Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
Impact factor: 5.924, year: 2020
Method of publishing: Open access
https://www.mdpi.com/1422-0067/21/7/2315

Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration. Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols. In this study, ALP-loaded gellan gum (GG) hydrogels were enriched with gallotannins, a subclass of polyphenols. Five preparations were compared, namely three tannic acids of differing molecular weight (MW), pentagalloyl glucose (PGG), and a gallotannin-rich extract from mango kernel (Mangifera indica L.). Certain gallotannin preparations promoted mineralization to a greater degree than others. The various gallotannin preparations bound differently to ALP and influenced the size of aggregates of ALP, which may be related to ability to promote mineralization. Human osteoblast-like Saos-2 cells grew in eluate from mineralized hydrogels. Gallotannin incorporation impeded cell growth on hydrogels and did not impart antibacterial activity. In conclusion, gallotannin incorporation aided mineralization but reduced cytocompatibility.
Permanent Link: http://hdl.handle.net/11104/0309999