The lipid code-dependent phosphoswitch PDK1–D6PK activates PIN-mediated auxin efflux in Arabidopsis

0531204 - ÚEB 2021 RIV GB eng J - Journal Article
Tan, S. - Zhang, X. - Kong, W. - Yang, X.-J. - Molnár, G. - Vondráková, Zuzana - Filepová, Roberta - Petrášek, Jan - Friml, J. - Xue, H.W.
The lipid code-dependent phosphoswitch PDK1–D6PK activates PIN-mediated auxin efflux in Arabidopsis.
Nature Plants. Roč. 6, č. 5 (2020), s. 556-569. ISSN 2055-026X. E-ISSN 2055-0278
Institutional support: RVO:61389030
Keywords : D6 PROTEIN-KINASE * PLASMA-MEMBRANE * PHOSPHATIDIC-ACID
OECD category: Biochemistry and molecular biology
Impact factor: 15.793, year: 2020
Method of publishing: Open access
http://doi.org/10.1038/s41477-020-0648-9

The PDK1 lipid-dependent kinase controls PIN1 and auxin transport through a phosphorylation cascade that includes AGC-type kinase D6PK. The double pdk1 mutant reveals auxin-related phenotypes such as reduced gravitropism and lateral roots. Directional intercellular transport of the phytohormone auxin mediated by PIN-FORMED (PIN) efflux carriers has essential roles in both coordinating patterning processes and integrating multiple external cues by rapidly redirecting auxin fluxes. PIN activity is therefore regulated by multiple internal and external cues, for which the underlying molecular mechanisms are not fully elucidated. Here, we demonstrate that 3 '-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE1 (PDK1), which is conserved in plants and mammals, functions as a molecular hub that perceives upstream lipid signalling and modulates downstream substrate activity through phosphorylation. Using genetic analysis, we show that the loss-of-function Arabidopsis pdk1.1 pdk1.2 mutant exhibits a plethora of abnormalities in organogenesis and growth due to defective polar auxin transport. Further cellular and biochemical analyses reveal that PDK1 phosphorylates D6 protein kinase, a well-known upstream activator of PIN proteins. We uncover a lipid-dependent phosphorylation cascade that connects membrane-composition-based cellular signalling with plant growth and patterning by regulating morphogenetic auxin fluxes.
Permanent Link: http://hdl.handle.net/11104/0309921