Root response in Pisum sativum under naproxen stress: Morpho-anatomical, cytological, and biochemical traits

0525486 - ÚVGZ 2020 RIV GB eng J - Journal Article
Svobodníková, L. - Kummerová, M. - Zezulka, Š. - Babula, P. - Sendecká, Katarína
Root response in Pisum sativum under naproxen stress: Morpho-anatomical, cytological, and biochemical traits.
Chemosphere. Roč. 258, NOV (2020), č. článku 127411. ISSN 0045-6535. E-ISSN 1879-1298
R&D Projects: GA ČR(CZ) GF17-33746L
Research Infrastructure: CzeCOS III - 90123
Institutional support: RVO:86652079
Keywords : Antioxidant defence * Non-steroidal anti-inflammatory drug * Oxidative stress * Products of transformation * Root system structure
OECD category: Plant sciences, botany
Impact factor: 7.086, year: 2020
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0045653520316052?via%3Dihub

Non-steroidal anti-inflammatory drugs as an important group of emerging environmental contaminants in irrigation water and soils can influence biochemical and physiological processes essential for growth and development in plants as non-target organisms. Plants are able to take up, transport, transform, and accumulate drugs in the roots. Root biomass in ten-days old pea plants was lowered by 6% already under 0.1 mg/L naproxen (NPX) due to a lowered number of lateral roots, although 0.5 mg/L NPX stimulated the total root length by 30% as against control. Higher section area (by 40%) in root tip, area of xylem (by 150%) or stele-to-section ratio (by 10%) in zone of maturation, and lower section area in zone of lateral roots (by 18%) prove the changes in primary root anatomy and its earlier differentiation at 10 mg/L NPX. Accumulated NPX (up to 10 μg/g DW at 10 mg/L) and products of its metabolization in roots increased the amounts of hydrogen peroxide (by 33%), and superoxide (by 62%), which was reflected in elevated lipid peroxidation (by 32%), disruption of membrane integrity (by 89%) and lowering both oxidoreductase and dehydrogenase activities (by up to 40%). Elevated antioxidant capacity (SOD, APX, and other molecules) under low treatments decreased at 10 mg/L NPX (both by approx. 30%). Naproxen was proved to cause changes at both cellular and tissue levels in roots, which was also reflected in their anatomy and morphology. Higher environmental loading through drugs thus can influence even the root function.
Permanent Link: http://hdl.handle.net/11104/0309898