Determination of Trolox equivalent antioxidant capacity in berries using amperometric tyrosinase biosensor based on multi-walled carbon nanotubes

0525437 - ÚMCH 2021 RIV CH eng J - Journal Article
Frangu, A. - Ashrafi, A. M. - Sýs, M. - Arbneshi, T. - Metelka, R. - Adam, V. - Vlček, Milan - Richtera, L.
Determination of Trolox equivalent antioxidant capacity in berries using amperometric tyrosinase biosensor based on multi-walled carbon nanotubes.
Applied Sciences-Basel. Roč. 10, č. 7 (2020), s. 1-13, č. článku 2497. E-ISSN 2076-3417
Institutional support: RVO:61389013
Keywords : amperometry * ascorbic acid * berries
OECD category: Inorganic and nuclear chemistry
Impact factor: 2.679, year: 2020
Method of publishing: Open access
https://www.mdpi.com/2076-3417/10/7/2497

In this contribution, Trolox equivalent antioxidant capacity (TEAC) was determined in various berries using carbon paste tyrosinase biosensor with multi-walled carbon nanotubes (MWCNTs), coated with Nafion® layer. Electrochemical behaviour of the biosensor and influence of MWCNTs on carbon paste surface were studied with respect to the sensitive amperometric detection of total content of phenolic compounds in berries, expressed as concentration equivalent of Trolox. After optimization of key instrumental and electroanalytical parameters, the biosensor was used for determination of TEAC in blackberries, blueberries, cranberries, raspberries and strawberries by method of multiple standard additions. Electrochemical TEAC assays corresponded well with results obtained by spectrophotometric 1,1-diphenyl-2-picrylhydrazyl radical method, known as DPPH assay. Obtained values were compared with those listed in the National Nutrient Database for additional antioxidant capacity assays as well.
Permanent Link: http://hdl.handle.net/11104/0309741