Evolution of multiple sex-chromosomes associated with dynamic genome reshuffling in Leptidea wood-white buttertflies

0524905 - BC 2021 RIV GB eng J - Journal Article
Yoshido, Atsuo - Šíchová, Jindra - Pospíšilová, Kristýna - Nguyen, Petr - Voleníková, Anna - Šafář, Jan - Provazník, Jan - Vila, R. - Marec, František
Evolution of multiple sex-chromosomes associated with dynamic genome reshuffling in Leptidea wood-white buttertflies.
Heredity. Roč. 125, č. 3 (2020), s. 138-154. ISSN 0018-067X. E-ISSN 1365-2540
R&D Projects: GA ČR(CZ) GA14-22765S; GA ČR(CZ) GA17-13713S; GA ČR(CZ) GA20-13784S
Grant - others:GA MŠk(CZ) LM2015042; GA ČR(CZ) GA17-17211S; GA ČR(CZ) GJ20-20650Y
Institutional support: RVO:60077344 ; RVO:61389030
Keywords : neo-sex chromosomes * BAC‐FISH mapping * speciation
OECD category: Genetics and heredity (medical genetics to be 3); Genetics and heredity (medical genetics to be 3) (UEB-Q)
Impact factor: 3.821, year: 2020
Method of publishing: Open access
https://www.nature.com/articles/s41437-020-0325-9.pdf

Sex-chromosome systems tend to be highly conserved and knowledge about their evolution typically comes from macroevolutionary inference. Rapidly evolving complex sex-chromosome systems represent a rare opportunity to study the mechanisms of sex-chromosome evolution at unprecedented resolution. Three cryptic species of wood-white butterflies—Leptidea juvernica, L. sinapis and L. reali—have each a unique set of multiple sex-chromosomes with 3–4 W and 3–4 Z chromosomes. Using a transcriptome-based microarray for comparative genomic hybridisation (CGH) and a library of bacterial artificial chromosome (BAC) clones, both developed in L. juvernica, we identified Z-linked Leptidea orthologs of Bombyx mori genes and mapped them by fluorescence in situ hybridisation (FISH) with BAC probes on multiple Z chromosomes. In all three species, we determined synteny blocks of autosomal origin and reconstructed the evolution of multiple sex-chromosomes. In addition, we identified W homologues of Z-linked orthologs and characterised their molecular differentiation. Our results suggest that the multiple sex-chromosome system evolved in a common ancestor as a result of dynamic genome reshuffling through repeated rearrangements between the sex chromosomes and autosomes, including translocations, fusions and fissions. Thus, the initial formation of neo-sex chromosomes could not have played a role in reproductive isolation between these Leptidea species. However, the subsequent species-specific fissions of several neo-sex chromosomes could have contributed to their reproductive isolation. Then, significantly increased numbers of Z-linked genes and independent neo-W chromosome degeneration could accelerate the accumulation of genetic incompatibilities between populations and promote their divergence resulting in speciation.
Permanent Link: http://hdl.handle.net/11104/0315117