Simple syntheses of new Pegylated trehalose derivatives as a chemical tool for potential evaluation of cryoprotectant effects on cell membrane

0524783 - FZÚ 2021 RIV CH eng J - Journal Article
Pomeisl, Karel - Richter, J. - Golan, Martin - Kratochvílová, Irena
Simple syntheses of new Pegylated trehalose derivatives as a chemical tool for potential evaluation of cryoprotectant effects on cell membrane.
Molecules. Roč. 25, č. 3 (2020), s. 1-14, č. článku 497. E-ISSN 1420-3049
R&D Projects: GA MŠMT EF16_013/0001406; GA MŠMT(CZ) LO1409
Grant - others:OP VVV - SAFMAT(XE) CZ.02.1.01/0.0/0.0/16_013/0001406
Institutional support: RVO:68378271
Keywords : click-chemistry * pegylation * cryoprotection
OECD category: Biophysics
Impact factor: 4.412, year: 2020
Method of publishing: Open access

In our work, we developed the synthesis of new polyfunctional pegylated trehalose derivatives and evaluated their cryoprotective effect using flow cytometry. We showed that new compounds (modified trehaloses) bound to appropriate extracellular polymeric cryoprotectants could be helpful as a chemical tool for the evaluation of their potential toxic cell membrane influences. Our aim was to form a chemical tool for the evaluation of cryoprotectant cell membrane influences, which are still not easily predicted during the freezing/thawing process. We combined two basic cryoprotectants: polyethyleneglycols (PEGs) and trehalose in the new chemical compounds—pegylated trehalose hybrids.

Permanent Link: http://hdl.handle.net/11104/0309075