IN VIVO BIODOSIMETRY OF PORCINE T-LYMPHOCYTE SUBSETS AND NK CELLS

0524744 - MBÚ 2021 RIV GB eng J - Journal Article
Andrejsova, L. - Šinkorová, Z. - Šinkora, J. - Tichý, A. - Filipová, A. - Němcová, M. - Šinkora, Marek
IN VIVO BIODOSIMETRY OF PORCINE T-LYMPHOCYTE SUBSETS AND NK CELLS.
Radiation Protection Dosimetry. Roč. 186, 2-3 (2020), s. 181-185. ISSN 0144-8420. E-ISSN 1742-3406
Institutional support: RVO:61388971
Keywords : radiation-induced apoptosis * total-body irradiation * blood b-cells
OECD category: Microbiology
Impact factor: 0.972, year: 2020
Method of publishing: Limited access
https://academic.oup.com/rpd/article-abstract/186/2-3/181/5704036

The aim of the present study was to evaluate the biodosimetric potential of peripheral blood lymphocytes, particularly of T-cell subsets (null and T helper) and natural killer cells (NK), upon exposure to gamma irradiation (Co-60) in vivo. For this purpose, the change in relative numbers of NK cells and T-lymphocyte subsets, as well as in the H2AX phosphorylation rate, were evaluated as potential early markers of the lymphocytic response to irradiation in vivo. These experiments were performed on a Large White Pig model. As a result, significant but not dose-dependent changes in the proportion of lymphocyte subpopulations (NK cells, null and T helper cells) were found after exposure to ionising radiation in vivo. On the other hand, circulating NK cells showed relatively higher radioresistance capacity when compared to the T-lymphocyte subsets, however, gamma-H2AX expression showed no significant difference between the evaluated lymphocyte subsets.
Permanent Link: http://hdl.handle.net/11104/0309046