Temperature‐programmed capillary high‐performance liquid chromatography with atmospheric pressure chemical ionization mass spectrometry for analysis of fatty acid methyl esters

0524555 - ÚOCHB 2021 RIV DE eng J - Journal Article
Vrkoslav, Vladimír - Rumlová, Barbora - Strmeň, Timotej - Cvačka, Josef
Temperature‐programmed capillary high‐performance liquid chromatography with atmospheric pressure chemical ionization mass spectrometry for analysis of fatty acid methyl esters.
Journal of Separation Science. Roč. 43, č. 13 (2020), s. 2579-2588. ISSN 1615-9306. E-ISSN 1615-9314
R&D Projects: GA ČR(CZ) GA16-01639S; GA MŠMT(CZ) EF16_019/0000729
Institutional support: RVO:61388963
Keywords : double bonds * high-performance liquid chromatography * lipidomics * lipids * temperature programming
OECD category: Analytical chemistry
Impact factor: 3.645, year: 2020
Method of publishing: Limited access
https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.201901235

A new capillary high‐performance liquid chromatography method with atmospheric pressure chemical ionization mass spectrometry was developed for the analysis of fatty acid methyl esters and long‐chain alcohols. The chromatographic separation was achieved using a Zorbax SB‐C18 HPLC column (0.3 × 150 mm, 3.5 μm) with a mobile phase composed of acetonitrile and formic acid and delivered isocratically at a flow rate of 10 μL/min. The column temperature was programmed simply, using a common column oven. Good reproducibility of the temperature profile and retention times were achieved. The temperature programming during the isocratic high‐performance liquid chromatography run had a similar effect as a solvent gradient, it reduced retention times of later eluting analytes and improved their detection limits. Two atmospheric pressure chemical ionization sources of the mass spectrometry detector were compared: an enclosed conventional ion source and an in‐house made ion source with a glass microchip nebulizer. The enclosed source provided better detectability of saturated fatty acid methyl esters and made it possible to determine the double bond positions using acetonitrile‐related adducts, while the open chip‐based source provided better analytical figures of merit for unsaturated fatty acid methyl esters. Temperature‐programmed capillary high‐performance liquid chromatography is a promising method for analyzing neutral lipids in lipidomics and other applications.
Permanent Link: http://hdl.handle.net/11104/0308895