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Chiroptical Properties and Conformation of Four Lasiocepsin-Related Antimicrobial Peptides: Structural Role of Disulfide Bridges

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    0524535 - ÚOCHB 2021 RIV CH eng J - Journal Article
    Pazderková, Markéta - Profant, V. - Maloň, P. - Dukor, R. K. - Čeřovský, Václav - Baumruk, V. - Bednárová, Lucie
    Chiroptical Properties and Conformation of Four Lasiocepsin-Related Antimicrobial Peptides: Structural Role of Disulfide Bridges.
    Symmetry-Basel. Roč. 12, č. 5 (2020), č. článku 812. E-ISSN 2073-8994
    R&D Projects: GA ČR GAP208/10/0376; GA ČR GAP205/10/1276
    Institutional support: RVO:61388963
    Keywords : antimicrobial peptides * lasiocepsin * electronic circular dichroism * vibrational circular dichroism * Raman optical activity * disulfide group
    OECD category: Biophysics
    Impact factor: 2.713, year: 2020
    Method of publishing: Open access
    https://www.mdpi.com/2073-8994/12/5/812

    We report an investigation of the role of disulfide bridges in the 27-residue antimicrobial peptide lasiocepsin (I) containing two disulfide groups (Cys8–Cys25, Cys17–Cys27) and three its analogs lacking one (II, III) or both (IV) native disulfides. Selective alternate incorporation of one or both disulfide bridges influences symmetry, conformation and biological properties of these peptides as demonstrated in their chiroptical (particularly Raman) properties. The effect of modifying the disulfide bridge pattern on the peptide secondary structure is investigated in water and in the presence of 2,2,2-trifluoroethanol and sodium dodecyl sulphate. A combination of experimental electronic and vibrational chiroptical data shows that both disulfide groups are necessary for stabilizing lasiocepsin secondary structure. While the Cys8–Cys25 disulfide group is important for sustaining lasiocepsin tertiary structure and maintaining its biological activity, the Cys17–Cys27 disulfide bridge has a supporting function consisting in reducing peptide flexibility.
    Permanent Link: http://hdl.handle.net/11104/0308881

     
     
Number of the records: 1