0524392 - ÚOCHB 2021 RIV NL eng J - Journal Article
Tůma, P. - Koval, Dušan - Sommerová, B. - Vaculín, Š.
Separation of anaesthetic ketamine and its derivates in PAMAPTAC coated capillaries with tuneable counter-current electroosmotic flow.
Talanta. Roč. 217, Sep (2020), č. článku 121094. ISSN 0039-9140. E-ISSN 1873-3573
R&D Projects: GA ČR(CZ) GA18-02597S
Institutional support: RVO:61388963
Keywords : clinical analysis * capillary electrophoresis * coating * contactless conductivity detection * Peak master * stacking
OECD category: Analytical chemistry
Impact factor: 6.057, year: 2020 ; AIS: 0.828, rok: 2020
Method of publishing: Limited access
Result website:
https://www.sciencedirect.com/science/article/pii/S0039914020303854?via%3DihubDOI: https://doi.org/10.1016/j.talanta.2020.121094

Capillary electrophoretic separation of ketamine, norketamine, hydroxynorketamine, and dehydronorketamine was performed in the counter-current regime under the influence of oppositely-directed electroosmotic flow. For this purpose, the fused silica capillaries were covalently coated with the poly(acrylamide-co-3-acrylamidopropyl trimethylammonium chloride) copolymer (PAMAPTAC). The content of the cationic monomer APTAC in the polymerization mixture varied in the range 0–6 mol. % and the generated electroosmotic flow increased continuously in the 0–20 · 10−9 m2V−1s−1 interval. Importantly, it resulted in improved electrophoretic resolution of ketamine/norketamine, which increased from 0.8 for neutral PAM coating (i.e. 0% PAMAPTAC) to 3.0 for 6% PAMAPTAC. The determination of ketamine and its derivates in rat serum was performed in a 4% PAMAPTAC capillary with an inner diameter of 25 μm. The separation was performed in a 500 mM aqueous solution of acetic acid (pH 2.3). The clinical sample was deproteinized by the addition of acetonitrile to the serum and a large volume of the treated sample was injected directly into the capillary. The achieved limit of detection ranged from 2.2 ng/mL for dehydronorketamine to 4.1 ng/mL for hydroxynorketamine, the intra-day repeatability was 1.0–1.5% for the migration time and 2.8–3.3% for the peak area. The developed methodology was employed for time monitoring of ketamines in rat serum after intra venous administration of low doses of anaesthetic at a level of 2 μg per g of body weight.
Permanent Link: http://hdl.handle.net/11104/0308748