Proteomic analysis of the cardiac myocyte secretome reveals extracellular protective functions for the ER stress response

0524331 - ÚIACH 2021 RIV GB eng J - Journal Article
Blackwood, E. A. - Thuerauf, D. J. - Šťastná, Miroslava - Stephens, H. - Sand, Z. - Pentoney, A. - Azizi, K. - Jakobi, T. - Van Eyk, J.E. - Katus, H. A. - Glembotski, Ch. C. - Doroudgar, S.
Proteomic analysis of the cardiac myocyte secretome reveals extracellular protective functions for the ER stress response.
Journal of Molecular and Cellular Cardiology. Roč. 143, JUN (2020), s. 132-144. ISSN 0022-2828. E-ISSN 1095-8584
Institutional support: RVO:68081715
Keywords : ER stress * cardioprotection * secreted proteins
OECD category: Analytical chemistry
Impact factor: 5.000, year: 2020
Method of publishing: Limited access

The effect of ER stress on protein secretion by cardiac myocytes using ER stressors thapsigargin (TG), which depletes ER calcium, and tunicamycin (TM) was studied. Proteomic analyses of TG- and TM-conditioned media showed that the secretion of most proteins was inhibited by TG and TM, however, secretion of several ER-resident proteins, including GRP78 was increased by TG but not TM. Simulated ischemia, which decreases SR/ER calcium also increased secretion of these proteins. The proteins secreted during ER stress mediated by ER calcium depletion can enhance cardiac myocyte viability.
Permanent Link: http://hdl.handle.net/11104/0308692