Upregulation of vitamin D-binding protein is associated with changes in insulin production in pancreatic beta-cells exposed to p,p '-DDT and p,p '-DDE

0524267 - MBÚ 2021 RIV GB eng J - Journal Article
Pavlíková, N. - Daniel, P. - Šrámek, J. - Jelínek, M. - Šrámková, V. - Němcová, V. - Balušíková, K. - Halada, Petr - Kovář, J.
Upregulation of vitamin D-binding protein is associated with changes in insulin production in pancreatic beta-cells exposed to p,p '-DDT and p,p '-DDE.
Scientific Reports. Roč. 9, DEC 2 (2019), č. článku 18026. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:61388971
Keywords : persistent organic pollutants * endoplasmic-reticulum stress * breast-cancer cells
OECD category: Biochemistry and molecular biology
Impact factor: 3.998, year: 2019
Method of publishing: Open access
https://d360prx.biomed.cas.cz:2645/articles/s41598-019-54579-z.pdf

Persistent organochlorine pollutants (POPs) gradually accumulate in the human organism due to their presence in the environment. Some studies have described a correlation between the level of POPs in the human body and the incidence of diabetes, but we know little about the direct effect of POPs on pancreatic beta-cells. We exposed pancreatic beta-cells INS1E to non-lethal concentrations of p,p'-DDT (1,1'-(2,2,2-Trichloroethane-1,1-diyl)bis(4-chlorobenzene)) and p,p'-DDE (1,1'-(2,2-dichloroethene-1,1-diyl)bis(4-chlorobenzene)) for 1 month, and assessed changes in protein expression and the intracellular insulin level. 2-D electrophoresis revealed 6 proteins with changed expression in cells exposed to p,p'-DDT or p,p'-DDE. One of the detected proteins - vitamin D-binding protein (VDBP) - was upregulated in both cells exposed to p,p'-DDT, and cells exposed to p,p'-DDE. Both exposures to pollutants reduced the intracellular level of insulin mRNA, proinsulin, and insulin monomer, p,p'-DDT also slightly reduced the level of hexameric insulin. Overexpression of VDBP caused by the stable transfection of beta-cells with the gene for VDBP decreased both the proinsulin and hexameric insulin level in beta-cells similarly to the reduction detected in cells exposed to p,p'-DDT. Our data suggest that in the cells exposed to p,p'-DDT and p,p'-DDE, the increased VDBP protein level decreased the proinsulin expression in an unknown mechanism.
Permanent Link: http://hdl.handle.net/11104/0308647