Interaction of GABA(A) and GABA(B) antagonists after status epilepticus in immature rats

0524266 - FGÚ 2021 RIV US eng J - Journal Article
Mareš, Pavel - Kubová, Hana
Interaction of GABA(A) and GABA(B) antagonists after status epilepticus in immature rats.
Epilepsy and Behavior. Roč. 102, Jan (2020), č. článku 106683. ISSN 1525-5050. E-ISSN 1525-5069
R&D Projects: GA ČR(CZ) GA15-16605S; GA ČR(CZ) GA18-09296S
Institutional support: RVO:67985823
Keywords : status epilepticus * convulsions * pentylenetetrazol * CGP46381 * immature rats
OECD category: Neurosciences (including psychophysiology
Impact factor: 2.937, year: 2020
Method of publishing: Limited access
https://doi.org/10.1016/j.yebeh.2019.106683

Among neurotransmitter systems affected by status epilepticus (SE) in adult rats are both GABAeigic systems. To analyze possible changes of GABA(A) and GABA(B) systems in developing rats lithium-pilocarpine SE was induced at postnatal day 12 (P12). Seizures were elicited by a GABA(A) antagonist pentylenetetrazol (PTZ) 3, 6, 9, and 13 days after SE (i.e., in P15, P18, P21, and P25 rats), and their possible potentiation by a GABA(B) receptor antagonist CGP46381 was studied. Pilocalpine was replaced by saline in control animals (lithium-paraldehyde [LiPAR]).Pentylenetetrazol in a dose of 50 mg/kg s.c. elicited generalized seizures in nearly all 15-day-old naive rats and in 40% of 18-day-old ones but not in older animals. After SE, PTZ no longer elicited seizures in these two younger groups, i.e., sensitivity of GABA(A) system was diminished. The GABA(B) antagonist exhibited proconvulsant effect in P15 and P18 SE as well as LiPAR rats returning the incidence of PTZ-induced seizures to values of control animals. A decrease in the incidence of minimal clonic seizures was seen in P21 LiPAR animals, these seizures in the oldest group were not affected. Change of the effect from proconvulsant to anliconvulsant (or at least to no action) took place before postnatal day 21.Both SE and LiPAR animals exhibited similar changes but their intensity differed, effects in LiPAR controls were usually more expressed than in SE rats.
Permanent Link: http://hdl.handle.net/11104/0308644