Electrographic seizures induced by activation of ETA and ETB receptors following intrahippocampal infusion of endothelin-1 in immature rats occur by different mechanisms

0524259 - FGÚ 2021 RIV US eng J - Journal Article
Vondráková, Kateřina - Novotný, P. - Kubová, Hana - Pošusta, Antonín - Boron, J. - Fáberová, Veronika - Fabene, P. F. - Burchfiel, J. - Tsenov, Grygoriy
Electrographic seizures induced by activation of ETA and ETB receptors following intrahippocampal infusion of endothelin-1 in immature rats occur by different mechanisms.
Experimental Neurology. Roč. 328, Jun (2020), č. článku 113255. ISSN 0014-4886. E-ISSN 1090-2430
R&D Projects: GA ČR(CZ) GA18-09296S; GA ČR(CZ) GA14-20613S
Institutional support: RVO:67985823
Keywords : ischemia * hippocampus * immature rat * endothelin * microdialysis, infmammatory * mass spektrometry * glutamate * GABA * leukotrienes * receptors * EEG * seizures
OECD category: Neurosciences (including psychophysiology
Impact factor: 5.330, year: 2020
Method of publishing: Limited access
https://doi.org/10.1016/j.expneurol.2020.113255

Using microdialysis we to determine biomarkers associated with seizures in P12 rats following the intrahippocampal infusion of two different agents: (1) ET-1, which activates both the ETA and ETB receptors and causes focal ischemia and (2) Ala-ET-1, which selectively activates only the ETB receptor and does not cause ischemia.
Our results show that seizures associated with combined ETA and ETB receptor activation (and ischemia) have a different temporal distribution and microdialysis profile from seizures associated with ETB activation alone (and without ischemia). Seizures induced with ETA and ETB activation peak within the first hour after infusion and the microdialysis profile is characterized by a significant increase in the ratio of glutamic acid to GABA. By contrast,
seizures with activation of only the ETB receptor peak in the second hour after infusion and microdialysis shows a significant increase in the ratio of leukotriene B4 to prostaglandin E2.
These findings suggest that ischemia-related seizures in immature animals involve an imbalance of excitation and inhibition, while non-ischemia related seizures involve an inflammatory process resulting from an excess of leukotrienes.

Permanent Link: http://hdl.handle.net/11104/0308641