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Catalytic and redox activity of nucleic acids at mercury electrodes: Roles of nucleobase residues

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    0524254 - BFÚ 2021 RIV CH eng J - Journal Article
    Římánková, Ludmila - Hasoň, Stanislav - Daňhel, Aleš - Fojta, Miroslav - Ostatná, Veronika
    Catalytic and redox activity of nucleic acids at mercury electrodes: Roles of nucleobase residues.
    Journal of Electroanalytical Chemistry. Roč. 858, FEB 1 2020 (2020), č. článku 113812. ISSN 1572-6657. E-ISSN 1873-2569
    R&D Projects: GA ČR(CZ) GA17-08971S; GA MŠk EF15_003/0000477
    Institutional support: RVO:68081707
    Keywords : hydrogen evolution * dna * electrochemistry * oligonucleotides
    OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
    Impact factor: 4.464, year: 2020
    Method of publishing: Limited access
    https://www.sciencedirect.com/science/article/pii/S157266571931080X?via%3Dihub

    Using a series of specifically designed oligonucleotides we have identified adenine and cytosine nucleobases as residues involved in catalytic hydrogen evolution reaction (CHER) of nucleic acids at the hanging mercury drop electrode (HMDE). Due to the CHER, nucleic acids yield catalytic peak H-NA allowing their label-free and reagent-less analysis at low concentrations. Additionally, our results suggest that presence of the electroactive bases (adenine and cytosine) facilitates guanine reduction which is for the first time linked to a signal measurable at the HMDE the peak P. We assume that the peak P is connected with reduction of guanine to 7,8-dihydroguanine, of which reoxidation to guanine is detected at the electrode by the earlier described anodic peak G. Processes connected with the above mentioned signals were studied using cyclic voltammetry by inspection of dependences on experimental parameters such as negative vertex potential and pH. (C) 2019 Elsevier B.V. All rights reserved.
    Permanent Link: http://hdl.handle.net/11104/0308636

     
     
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