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Study of permeabilization of bacterial membrane by electrochemical methods

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    0524215 - ÚFCH JH 2021 RIV CH eng J - Journal Article
    Čierna, M. - Naumowicz, M. - Birošová, L. - Krahulec, J. - Sokolová, Romana - Kolivoška, Viliam - Sebechlebská, Táňa - Kielar, F. - Gál, M.
    Study of permeabilization of bacterial membrane by electrochemical methods.
    Journal of Electroanalytical Chemistry. Roč. 857, JAN 2020 (2020), č. článku 113761. ISSN 1572-6657. E-ISSN 1873-2569
    R&D Projects: GA ČR(CZ) GA18-09848S
    Institutional support: RVO:61388955
    Keywords : antimicrobial peptides * escherichia-coli * carbon nutrition * evolution * ll-37 * Voltammetry * Cathelicidin * Permeabilization * E. coli
    OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
    Impact factor: 4.464, year: 2020
    Method of publishing: Open access

    Selected electrochemical methods for the determination of possible membrane permeabilization in two Escherichia coli bacterial strains were examined. Escherichia coli, a gram-negative rod-shaped bacterium, is an important component of the mammalian intestinal microbiota. However, some strains of this bacterium are pathogenic and can cause a variety of diseases. Partial aim of this work was to identify beta-galactosidase enzyme as one of the possible factors characterizing disintegration of bacterial membrane. To permeabilize the bacterial membrane, cathelicidin LL-37, the body's own antimicrobial peptide, and membrane degradation by microwave radiation were used. In the investigation of structural changes in the bacterial membrane, square-wave voltammetry and chronopotentiometric dissolution analysis in combination with the mercury drop electrode and the glassy carbon electrode were used. UV/VIS spectrophotometry was used to indirectly track changes in bacterial cell structure. (C) 2019 The Authors. Published by Elsevier B.V.
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