Interleaflet Coupling of Lipid Nanodomains – Insights From in vitro Systems

0524199 - ÚFCH JH 2021 RIV CH eng J - Journal Article
Sarmento, Maria Joäo - Hof, Martin - Šachl, Radek
Interleaflet Coupling of Lipid Nanodomains – Insights From in vitro Systems.
Frontiers in Cell and Developmental Biology. Roč. 8, APR 2020 (2020), č. článku 284. ISSN 2296-634X. E-ISSN 2296-634X
R&D Projects: GA ČR(CZ) GX19-26854X; GA ČR(CZ) GA18-04871S; GA MŠMT(CZ) EF16_027/0008355
Institutional support: RVO:61388955
Keywords : domain registration * interleaftet coupling * membrane asymmetry
OECD category: Physical chemistry
Impact factor: 6.684, year: 2020
Method of publishing: Open access

The plasma membrane is a complex system, consisting of two layers of lipids and proteins compartmentalized into small structures called nanodomains. Despite the asymmetric composition of both leaflets, coupling between the layers is surprisingly strong. This can be evidenced, for example, by recent experimental studies performed on phospholipid giant unilamellar vesicles showing that nanodomains formed in the outer layer are perfectly registered with those in the inner leaflet. Similarly, microscopic phase separation in one leaflet can induce phase separation in the opposing leaflet that would otherwise be homogeneous. In this review, we summarize the current theoretical and experimental knowledge that led to the current view that domains are – irrespective of their size – commonly registered across the bilayer. Mechanisms inducing registration of nanodomains suggested by theory and calculations are discussed. Furthermore, domain coupling is evidenced by experimental studies based on the sparse number of methods that can resolve registered from independent nanodomains. Finally, implications that those findings using model membrane studies might have for cellular membranes are discussed.
Permanent Link: http://hdl.handle.net/11104/0308581