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Lipokine 5-PAHSA is Regulated by Adipose Triglyceride Lipase and Primes Adipocytes for de novo Lipogenesis in Mice

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    0524172 - FGÚ 2021 RIV US eng J - Journal Article
    Palůchová, Veronika - Oseeva, Marina - Březinová, Marie - Čajka, Tomáš - Bardová, Kristina - Adamcová, Kateřina - Žáček, P. - Brejchová, Kristýna - Balas, L. - Chodounská, Hana - Kudová, Eva - Schreiber, R. - Zechner, R. - Durand, T. - Rossmeisl, Martin - Abumrad, N. A. - Kopecký, Jan - Kuda, Ondřej
    Lipokine 5-PAHSA is Regulated by Adipose Triglyceride Lipase and Primes Adipocytes for de novo Lipogenesis in Mice.
    Diabetes. Roč. 69, č. 3 (2020), s. 300-312. ISSN 0012-1797. E-ISSN 1939-327X
    R&D Projects: GA ČR(CZ) GJ17-10088Y; GA MŠMT(CZ) LTAUSA17173; GA MŠMT(CZ) LTAUSA18104; GA MŠMT(CZ) ED1.1.00/02.0109
    Grant - others:AV ČR(CZ) LQ200111901
    Program: Prémie Lumina quaeruntur
    Institutional support: RVO:67985823 ; RVO:61388963
    Keywords : PAHSA * FAHFA * type 2 diabetes * metabolic flux analysis * glucose metabolism
    OECD category: Endocrinology and metabolism (including diabetes, hormones); Neurosciences (including psychophysiology (UOCHB-X)
    Impact factor: 9.461, year: 2020
    Method of publishing: Limited access
    https://doi.org/10.2337/db19-0494

    Branched esters of palmitic acid and hydroxy-stearic acid (PAHSA) are anti-inflammatory and anti-diabetic lipokines that connect glucose and lipid metabolism. We aimed to characterize involvement of the 5-PAHSA regioisomer in the adaptive metabolic response of white adipose tissue (WAT) to cold exposure (CE) in mice, exploring the crosstalk between glucose utilization and lipid metabolism. CE promoted local production of 5- and 9-PAHSAs in WAT. Metabolic labeling of de novo lipogenesis (DNL) using 2H2O revealed that 5-PAHSA potentiated the effects of CE and stimulated triacylglycerol/fatty acid (TAG/FA) cycling in WAT through impacting lipogenesis and lipolysis. Adipocyte lipolytic products were altered by 5-PAHSA through selective FA re-esterification. The impaired lipolysis in global adipose triglyceride lipase (ATGL) knockout mice reduced free PAHSA levels and uncovered a metabolite reservoir of TAG-bound PAHSAs (TAG-estolides) in WAT. Utilization of 13C isotope tracers and dynamic metabolomics documented that 5-PAHSA primed adipocytes for glucose metabolism in a different way from insulin, promoting DNL, and impeding TAG synthesis. In summary, our data revealed new cellular and physiological mechanisms underlying the beneficial effects of 5-PAHSA, its relation to insulin action in adipocytes, and independently confirmed a PAHSA metabolite reservoir linked to ATGL-mediated lipolysis.
    Permanent Link: http://hdl.handle.net/11104/0308535

     
     
Number of the records: 1  

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