Online Concentration of Bacteria from Tens of Microliter Sample Volumes in Roughened Fused Silica Capillary with Subsequent Analysis by Capillary Electrophoresis and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

0524062 - ÚIACH 2021 RIV US eng J - Journal Article
Horká, Marie - Šalplachta, Jiří - Karásek, Pavel - Růžička, F. - Roth, Michal
Online Concentration of Bacteria from Tens of Microliter Sample Volumes in Roughened Fused Silica Capillary with Subsequent Analysis by Capillary Electrophoresis and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.
ACS Infectious Diseases. Roč. 6, č. 3 (2020), s. 355-365. ISSN 2373-8227
R&D Projects: GA ČR(CZ) GA19-00742S; GA MZd(CZ) NV16-29916A; GA MV VI20172020069
Institutional support: RVO:68081715
Keywords : nano-etched FS capillary * CE and MALDI methods * cell-surface adhesion from blood samples
OECD category: Analytical chemistry
Impact factor: 5.084, year: 2020
Method of publishing: Limited access

The cells of S. aureus (methicillin susceptible or methicillin-resistant) and of P. aeruginosa were adhered onto nano-etched FS surface prepared by etching with SCW from 100 mL physiological saline solution, bovine serum, or human blood, and separated on-line by MEKC and off-line by MALDI-TOF MS. The limits of detection was from 180 to 1700 cells mL−1 at MEKC analysis and 4000 cells mL−1 at MALDI-TOF MS.
Permanent Link: http://hdl.handle.net/11104/0308397