The Formation of Sex Chromosomes in Silene latifolia and S. dioica Was Accompanied by Multiple Chromosomal Rearrangements

0523827 - ÚEB 2021 RIV CH eng J - Journal Article
Bačovský, V. - Čegan, Radim - Šimoníková, Denisa - Hřibová, Eva - Hobza, Roman
The Formation of Sex Chromosomes in Silene latifolia and S. dioica Was Accompanied by Multiple Chromosomal Rearrangements.
Frontiers in Plant Science. Roč. 11, FEB 28 (2020), č. článku 205. ISSN 1664-462X. E-ISSN 1664-462X
R&D Projects: GA ČR(CZ) GA18-06147S
Institutional support: RVO:61389030
Keywords : chromosome painting * double-translocation * pseudo-autosomal region * Silene * Y chromosome
OECD category: Plant sciences, botany
Impact factor: 5.754, year: 2020
Method of publishing: Open access
http://doi.org/10.3389/fpls.2020.00205

The genus Silene includes a plethora of dioecious and gynodioecious species. Two species, Silene latifolia (white campion) and Silene dioica (red campion), are dioecious plants, having heteromorphic sex chromosomes with an XX/XY sex determination system. The X and Y chromosomes differ mainly in size, DNA content and posttranslational histone modifications. Although it is generally assumed that the sex chromosomes evolved from a single pair of autosomes, it is difficult to distinguish the ancestral pair of chromosomes in related gynodioecious and hermaphroditic plants. We designed an oligo painting probe enriched for X-linked scaffolds from currently available genomic data and used this probe on metaphase chromosomes of S. latifolia (2n = 24, XY), S. dioica (2n = 24, XY), and two gynodioecious species, S. vulgaris (2n = 24) and S. maritima (2n = 24). The X chromosome-specific oligo probe produces a signal specifically on the X and Y chromosomes in S. latifolia and S. dioica, mainly in the subtelomeric regions. Surprisingly, in S. vulgaris and S. maritima, the probe hybridized to three pairs of autosomes labeling their p-arms. This distribution suggests that sex chromosome evolution was accompanied by extensive chromosomal rearrangements in studied dioecious plants.
Permanent Link: http://hdl.handle.net/11104/0308109