The bio-chemically selective interaction of hydrogenated and oxidized ultra-small nanodiamonds with proteins and cells

0523791 - ÚOCHB 2021 RIV US eng J - Journal Article
Machová, I. - Hubálek, Martin - Bělinová, T. - Fučíková, A. - Stehlík, Štěpán - Rezek, B. - Hubálek Kalbáčová, M.
The bio-chemically selective interaction of hydrogenated and oxidized ultra-small nanodiamonds with proteins and cells.
Carbon. Roč. 162, Jun (2020), s. 650-661. ISSN 0008-6223. E-ISSN 1873-3891
R&D Projects: GA MŠMT(CZ) EF16_019/0000760; GA ČR(CZ) GJ18-11711Y; GA MŠMT LM2015087; GA MŠMT(CZ) LM2015047
Institutional support: RVO:61388963 ; RVO:68378271
Keywords : fetal bovine serum * gold nanoparticles * surface chemistry
OECD category: Biochemistry and molecular biology
Impact factor: 9.594, year: 2020
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/abs/pii/S0008622320302074

Ultra-small nanoparticles of a size smaller than or comparable to cell membrane pores (1–5 nm) offer significant potential in the field of biomedicine. This study presents a systematic in vitro investigation of fundamental bio-chemical interactions of such ultra-small hydrogenated and oxidized detonation nanodiamonds (DNDs) with biomolecules and human cells. We apply mass spectrometry methods (LC-MS/MS) for the qualitative and quantitative analysis of the protein corona as a function of the surface chemistry and size of DNDs. We observe that protein interactions with DNDs are more related to their surface chemistry (H/O-termination) rather than size. Bioinformatics characterization of the identified proteins points to the strong influence of electrostatic interaction between proteins and DNDs depending on their termination. Such specific interaction leads to formation of different protein corona on 2 nm DNDs, which influences also interaction with cells including different level of cytotoxicity.
Permanent Link: http://hdl.handle.net/11104/0308084