Anti-proliferative activity of a novel tricyclic triterpenoid acid from Commiphora africana resin against four human cancer cell lines

0523785 - ÚOCHB 2021 RIV SG eng J - Journal Article
Dinku, W. - Isaksson, J. - Rylandsholm, F. G. - Bouř, Petr - Brichtová, Eva - Choi, S. U. - Lee, S. H. - Jung, Y. S. - No, Z. S. - Svendsen, J. S. M. - Aasen, A. J. - Dekebo, A.
Anti-proliferative activity of a novel tricyclic triterpenoid acid from Commiphora africana resin against four human cancer cell lines.
Applied Biological Chemistry. Roč. 63, č. 1 (2020), č. článku 16. ISSN 2468-0834. E-ISSN 2468-0842
R&D Projects: GA ČR(CZ) GA18-05770S; GA MŠMT(CZ) LTC17012; GA MŠMT(CZ) EF16_019/0000729
Institutional support: RVO:61388963
Keywords : Commiphora africana * Commafric A * anti-proliferative * SRB * A549 cell line
OECD category: Biophysics
Impact factor: 1.813, year: 2020
Method of publishing: Open access
https://applbiolchem.springeropen.com/articles/10.1186/s13765-020-00499-w

Myrrh, a resin derived from the damaged bark of Commiphora genus, has traditionally been used for treatment of various human diseases, such as amenorrhea, ache, tumors, fever, and stomach pains. In spite of this widespread use of the myrrh in Ethiopia, the pharmacological activity and chemical composition have not been studied in detail. A new tricyclic triterpene acid (3S,4S,14S,7E,17E,21Z)-3,30-dihydroxypodioda-7,17,21-trien-4-carboxylic acid (commafric A) has been isolated from a crude methanolic extract of Commiphora africana (A. Rich.) Engl. resin along with the known pentacyclic triterpene alpha-amyrin. The structure of commafric A was characterized using different spectroscopic techniques such as 1D and 2D NMR, IR, and VCD combined with computations. The anti-proliferative activity of both isolated compounds was evaluated using SRB based colorimetric cellular assay against four human cancer cell lines. Etoposide was used as a positive control. Commafric A showed significant anti-proliferative effects against non-small cell lung cancer (A549) with IC50 values of 4.52 mu g/ml. The pentacyclic triterpene alpha-amyrin showed a weak anti-proliferative activity against A2780 (ovarian cancer), MIA-PaCa-2 (pancreatic cancer), and SNU638 (stomach cancer) cell lines tested with IC50 values ranging 9.28 to 28.22 mu g/ml. Commafric A possessed anti-proliferative activity against non-small cell lung cancer (A549), which suggests that commafric A has potential to be further optimized being a lead compound in the search for new drugs against cancer diseases.
Permanent Link: http://hdl.handle.net/11104/0308082