Electrochemical characterization of the artificial metalloenzyme papain-[(ηsup6/sup-arene)Ru(1,10-phenanthroline)Cl]sup+/sup

0523422 - ÚFCH JH 2021 RIV CH eng J - Journal Article
Nováková Lachmanová, Štěpánka - Pospíšil, Lubomír - Šebera, Jakub - Talbi, B. - Salmain, M. - Hromadová, Magdaléna
Electrochemical characterization of the artificial metalloenzyme papain-[(ηsup6/sup-arene)Ru(1,10-phenanthroline)Cl]sup+/sup.
Journal of Electroanalytical Chemistry. Roč. 859, FEB 2020 (2020), č. článku 113882. ISSN 1572-6657. E-ISSN 1873-2569
R&D Projects: GA ČR(CZ) GA18-04682S; GA MŠMT(CZ) 8J18FR002
Grant - others:Akademie věd - GA AV ČR(CZ) L2004020020
Institutional support: RVO:61388955
Keywords : Artificial metalloenzyme * Electrochemical admittance and impedance techniques * Papain * Ruthenium(II) complexes
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Impact factor: 4.464, year: 2020
Method of publishing: Limited access

Electrochemical properties were studied for [(η6-arene)Ru(1,10-phenanthroline)Cl]Cl (arene = C6H5(CH2)2NHCOCH2Cl) organometallic complex 1, protein Papain PAP and its conjugate with organometallic complex 1-PAP. The latter can serve as an artificial metalloenzyme with catalytic activity in transfer hydrogenation. This work demonstrates that AC voltammetry and electrochemical impedance spectroscopy can be used as fast tools to screen the catalytic ability of 1-PAP electrochemically by studies of the catalytic hydrogen evolution reaction (HER). Proteins are known to catalyze this process, but we have shown that additional HER signal associated with the catalytic activity of 1 is observed for its conjugate with Papain 1-PAP.
Permanent Link: http://hdl.handle.net/11104/0307779