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Advances in Disease Models
- 1.0523242 - ÚMG 2020 RIV CZ eng M - Monography Chapter
Schuster, Bjorn - Epp, Trevor - Chawengsaksophak, Kallayanee
Efficient generation of transgenic animal models using in vivo electroporation.
Advances in Disease Models. Praha: OPTIO CZ, 2019 - (Bartůněk, P.), s. 226-233. ISBN 978-80-88011-06-4
R&D Projects: GA MŠMT LO1419; GA ČR GA17-16959S
Institutional support: RVO:68378050
Keywords : CRISPR/Cas * in vivo electroporation * iGONAD
OECD category: Biochemistry and molecular biology
The generation of animal model systems to study protein function or to mimic human genetic diseases is a valuable tool in biomedical research. Using traditional methods for the model generation requires expensive equipment and is time consuming. Recently, the CRISPR/Cas technology not only improved the efficiency of genomic manipulations, it also opened up new ways for the delivery of the reagents to the cells. In this book chapter we describe the iGONAD method allowing targeted mouse model production applicable for a wide range of research labs to introduce the desired genomic modifications without the need of special equipment.
Permanent Link: http://hdl.handle.net/11104/0307614
Number of the records: 1