0523226 - ÚMG 2020 RIV CZ eng M - Monography Chapter
Epp, Trevor - Schuster, Bjorn - Chawengsaksophak, Kallayanee
Development of a novel gene mutagenesis and protein tagging strategy.
Advances in Disease Models. Praha: OPTIO CZ, 2019 - (Bartůněk, P.), s. 106-114. ISBN 978-80-88011-06-4
R&D Projects: GA MŠMT LO1419; GA ČR GA17-16959S
Institutional support: RVO:68378050
Keywords : protein tagging strategy * novel gene mutagenesis * RNA splicing
OECD category: Biochemistry and molecular biology

Random gene trap mutagenesis is a powerful technique to rapidly generate a library of mutated genes while also providing a reporter for their respective expression. One problem is that efficient mutagenesis requires insertion of the vector at the beginning of a gene, while an informative subcellular reporter requires insertion at the end of a gene. Here we introduce a novel gene trap approach that overcomes this issue, creating a multifunctional allele that permits both effective mutagenesis and endogenous protein tagging.
Permanent Link: http://hdl.handle.net/11104/0307601