THE EFFECT OF SIMULATED BODY FLUID ON THE GROWTH AND DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS CULTURED ON NANOSTRUCTURED Ti6Al4V SURFACES

0522951 - ÚEM 2020 RIV CZ eng C - Conference Paper (international conference)
Filová, Eva - Voltrová, Barbora - Jarolímová, P. - Matějka, Roman - Lukášová, Věra - Fojt, J. - Joska, L. - Daniel, M.
THE EFFECT OF SIMULATED BODY FLUID ON THE GROWTH AND DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS CULTURED ON NANOSTRUCTURED Ti6Al4V SURFACES.
NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application. Ostrava: Tanger Ltd., 2019, s. 300-305. ISBN 978-808729489-5.
[NANOCON 2018 -International Conference on Nanomaterials - Research and Application /10./. Brno (CZ), 17.10.2018-19.10.2018]
R&D Projects: GA MŠMT(CZ) LO1508; GA ČR(CZ) GA16-14758S
Institutional support: RVO:68378041 ; RVO:67985823
Keywords : anodic oxidation * hydroxyapatite * mesenchymal stem cell
OECD category: Biomaterials (as related to medical implants, devices, sensors); Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction) (FGU-C)

TI6Al4V is a standard medical titanium alloy. In this study, we compared ground Ti6Al4V discs with nanostructured Ti6Al4V discs prepared using the anodic oxidation method at 30V. Subsequently, both the nanostructured and ground discs were exposed to simulated body fluid (SBF) for 7 days in order to form a hydroxyapatite-like layer. Human mesenchymal stem cells (hMSC) were seeded on the discs in osteogenic differentiation medium and cultivated for 21 days. hMSCs were tested for metabolic activity (MTS assay) and rate of proliferation. The synthesis of type I collagen was evaluated using immunohistochemical staining and confocal microscopy analysis. All samples supported cell adhesion and proliferation. The highest amount of type I collagen was found on nanostructured (N) and ground Ti6Al4V exposed to SBF (TiH) surfaces. Thus, SBF and anodic oxidation positively influenced the growth and osteogenic differentiation of hMSCs.
Permanent Link: http://hdl.handle.net/11104/0307357