Taste for protein: Chemical signal from prey stimulates enzyme secretion through jasmonate signalling in the carnivorous plant Venus flytrap

0522617 - ÚEB 2020 RIV FR eng J - Journal Article
Jakšová, J. - Libiaková, M. - Bokor, B. - Petřík, Ivan - Novák, Ondřej - Pavlovič, A.
Taste for protein: Chemical signal from prey stimulates enzyme secretion through jasmonate signalling in the carnivorous plant Venus flytrap.
Plant Physiology and Biochemistry. Roč. 146, January (2020), s. 90-97. ISSN 0981-9428
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : Action potential * Carnivorous plant * Chitinase * Digestive enzyme * Jasmonic acid * Protease * Venus flytrap
OECD category: Biochemistry and molecular biology
Impact factor: 4.270, year: 2020
Method of publishing: Open access
https://doi.org/10.1016/j.plaphy.2019.11.013

Hunting cycle of the carnivorous plant Venus flytrap (Dionaea muscipula Ellis) is comprised of mechanism for rapid trap closure followed by slow hermetical sealing and activation of gene expression responsible for digestion of prey and nutrient uptake. In the present study, we focus on the late phase of Venus's flytrap hunting cycle when mechanical stimulation of the prey ceases and is replaced by chemical cues. We used two nitrogen-rich compounds (chitin and protein) in addition to mechanostimulation to investigate the electrical and jasmonate signalling responsible for induction of enzyme activities. Chemical stimulation by BSA protein and chitin did not induce any additional spontaneous action potentials (APs). However, chemical stimulation by protein induced the highest levels of jasmonic acid (JA) and its isoleucine conjugate (JA-Ile) as well as the expression of studied gene encoding a cysteine protease (dionain). Although chitin is probably the first chemical agent which is in direct contact with digestive glands, presence of protein in the secured trap mimics the presence of insect prey best.
Permanent Link: http://hdl.handle.net/11104/0307082