Comparison of 12 DNA extraction kits for vertebrate samples

0522567 - ÚBO 2021 RIV ES eng J - Journal Article
Martincová, Iva - Aghová, Tatiana
Comparison of 12 DNA extraction kits for vertebrate samples.
Animal Biodiversity And Conservation. Roč. 43, č. 1 (2020), s. 67-77. ISSN 1578-665X. E-ISSN 2014-928X
Institutional support: RVO:68081766
Keywords : DNA concentration * DNA isolation * DNA purity * Price of extraction kit
OECD category: Biodiversity conservation
Impact factor: 1.000, year: 2020
Method of publishing: Open access
https://doi.org/10.32800/abc.2020.43.0067

Papers are published under a. Obtaining high quality DNA extractions is a crucial step for molecular biology research projects. At present, numerous protocols are available for vertebrate tissue extractions. In the present study we compared eleven column–based protocols and one HotSHOT protocol using similar conditions (i.e., type of sample, weight of starting material). We evaluated time of extraction, quality and quantity of DNA yield, and price of extraction for a single sample. Based on our analysis, the most successful kits for producing DNA with the highest concentration and purity are the JetQuick® Genomic DNA Purification Kit (Genomed) and the NucleoSpin® Tissue (Macherey–Nagel). Nevertheless, it is highly recommended to test various extraction kits with specific samples to find the optimal kit in all aspects of time, quality and cost for a particular project.
Permanent Link: http://hdl.handle.net/11104/0307038