The effect of mitochondrially targeted anticancer agents on mitochondrial (super)complexes

0522123 - BTÚ 2020 RIV US eng J - Journal Article
Vondrusová, Magdaléna - Bezawork-Geleta, A. - Sachaphibulkij, K. - Truksa, Jaroslav - Neužil, Jiří
The effect of mitochondrially targeted anticancer agents on mitochondrial (super)complexes.
Methods in molecular biology. Roč. 1265, č. 2015 (2015), s. 195-208. ISSN 1940-6029
Institutional support: RVO:86652036
Keywords : antineoplastic agent * mitochondrial protein * multienzyme complex
OECD category: Biochemistry and molecular biology
Method of publishing: Limited access
https://link.springer.com/protocol/10.1007%2F978-1-4939-2288-8_15

The mitochondrial respiratory chain is organized into dynamic high molecular weight complexes that associate to form supercomplexes. The function of these SCs is to minimize the production of reactive oxygen species (ROS) generated during electron transfer within them and to efficiently transfer electrons to complex IV. These supra-molecular structures as well as whole mitochondria are stress-responsive and respond to mitochondrially targeted anti-cancer agent by destabilization and induction of massive production of ROS leading to apoptosis. We have recently developed mitochondrially targeted anti-cancer agents epitomized by the mitochondrially targeted analogue of the redox-silent compound vitamin E succinate, which belongs to the group of agents that kill cancer cells via their mitochondria-destabilizing activity, referred to as mitocans. To understand the molecular mechanism of the effect of such agents, the use of native blue gel electrophoresis and clear native electrophoresis coupled with in-gel activity assays, are methods of choice. The relevant methodology is described in this chapter.
Permanent Link: http://hdl.handle.net/11104/0306632