Deciphering retinal diseases through the generation of three dimensional stem cell-derived organoids: Concise Review

0520812 - ÚEM 2020 RIV US eng J - Journal Article
Artero-Castro, A. - Rodríguez, J.F.J. - Jendelová, Pavla - Erceg, Slaven
Deciphering retinal diseases through the generation of three dimensional stem cell-derived organoids: Concise Review.
Stem Cells. Roč. 37, č. 12 (2019), s. 1496-1504. ISSN 1066-5099. E-ISSN 1549-4918
R&D Projects: GA ČR(CZ) GA18-04393S; GA MŠMT(CZ) EF15_003/0000419
Institutional support: RVO:68378041
Keywords : 3D organoids * disease modeling * induced pluripotent stem cells
OECD category: Physiology (including cytology)
Impact factor: 6.022, year: 2019
Method of publishing: Open access
https://stemcellsjournals.onlinelibrary.wiley.com/doi/full/10.1002/stem.3089

Three-dimensional (3D) retinal organoids, in vitro tissue structures derived from self-organizing cultures of differentiating human embryonic stem cells or induced pluripotent stem cells, could recapitulate some aspects of the cytoarchitectural structure and function of the retina in vivo. 3D retinal organoids display huge potential for the investigation of the pathogenesis of monogenic hereditary eye diseases that are related to the malfunction or degeneration of photoreceptors or retinal ganglion cells by providing an effective in vitro tool with multiple applications. In combination with recent genome editing tools, 3D retinal organoids could also represent a reliable and renewable source of transplantable cells for personalized therapies. In this review, we describe the recent advances in human pluripotent stem cells-derived retinal organoids, determination of their histoarchitecture, complexity, and maturity. We also discuss their application as a means to decipher the pathogenesis of retinal diseases, as well as the main drawbacks and challenges.
Permanent Link: http://hdl.handle.net/11104/0305477