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Characterization of Aminoacyl-tRNA Synthetases in Chromerids

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    0520511 - BC 2020 RIV CH eng J - Journal Article
    Sharaf, Abdoallah - Gruber, Ansgar - Jiroutová, Kateřina - Oborník, Miroslav
    Characterization of Aminoacyl-tRNA Synthetases in Chromerids.
    Genes. Roč. 10, č. 8 (2019), č. článku 582. E-ISSN 2073-4425
    R&D Projects: GA MŠMT(CZ) EF16_019/0000759; GA ČR GA15-17643S
    Institutional support: RVO:60077344
    Keywords : phototrophic relatives * thermus-thermophilus * mitochondrial genome * glutamine codons * signal peptides * apicomplexan * dinoflagellate * evolution * acid * purification * Chromera velia * Vitrella brassicaformis * Aminoacyl tRNA synthetase (AaRS) * evolution * protein localization * chloroplast * mitochondrion * nucleus
    OECD category: Microbiology
    Impact factor: 3.759, year: 2019
    Method of publishing: Open access
    https://www.mdpi.com/2073-4425/10/8/582

    Aminoacyl-tRNA synthetases (AaRSs) are enzymes that catalyze the ligation of tRNAs to amino acids. There are AaRSs specific for each amino acid in the cell. Each cellular compartment in which translation takes place (the cytosol, mitochondria, and plastids in most cases), needs the full set of AaRSs, however, individual AaRSs can function in multiple compartments due to dual (or even multiple) targeting of nuclear-encoded proteins to various destinations in the cell. We searched the genomes of the chromerids, Chromera velia and Vitrella brassicaformis, for AaRS genes: 48 genes encoding AaRSs were identified in C. velia, while only 39 AaRS genes were found in V. brassicaformis. In the latter alga, ArgRS and GluRS were each encoded by a single gene occurring in a single copy, only PheRS was found in three genes, while the remaining AaRSs were encoded by two genes. In contrast, there were nine cases for which C. velia contained three genes of a given AaRS (45% of the AaRSs), all of them representing duplicated genes, except AsnRS and PheRS, which are more likely pseudoparalogs (acquired via horizontal or endosymbiotic gene transfer). Targeting predictions indicated that AaRSs are not (or not exclusively), in most cases, used in the cellular compartment from which their gene originates. The molecular phylogenies of the AaRSs are variable between the specific types, and similar between the two investigated chromerids. While genes with eukaryotic origin are more frequently retained, there is no clear pattern of orthologous pairs between C. velia and V. brassicaformis.
    Permanent Link: http://hdl.handle.net/11104/0305174

     
     
Number of the records: 1  

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