Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin

0520507 - BC 2020 RIV US eng J - Journal Article
Selinger, Martin - Tykalová, Hana - Štěrba, Ján - Věchtová, Pavlína - Vavrušková, Zuzana - Lieskovská, J. - Kohl, A. - Schnettler, E. - Grubhoffer, Libor
Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin.
PLoS Neglected Tropical Diseases. Roč. 13, č. 9 (2019), č. článku e0007745. ISSN 1935-2735. E-ISSN 1935-2735
R&D Projects: GA MŠMT(CZ) LTARF18021; GA ČR(CZ) GA18-27204S; GA MŠMT(CZ) LM2015055
Institutional support: RVO:60077344
Keywords : translation initiation * high-virulence * interferon * infection * viperin * establishment * replication * cleavage * regions * line
OECD category: Microbiology
Impact factor: 3.885, year: 2019
Method of publishing: Open access
https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0007745

Tick-borne encephalitis virus (TBEV), a member of the genus Flavivirus (Flaviviridae), is a causative agent of a severe neuroinfection. Recently, several flaviviruses have been shown to interact with host protein synthesis. In order to determine whether TBEV interacts with this host process in its natural target cells, we analysed de novo protein synthesis in a human cell line derived from cerebellar medulloblastoma (DAOY HTB-186). We observed a significant decrease in the rate of host protein synthesis, including the housekeeping genes HPRT1 and GAPDH and the known interferon-stimulated gene viperin. In addition, TBEV infection resulted in a specific decrease of RNA polymerase I (POLR1) transcripts, 18S and 28S rRNAs and their precursor, 45-47S pre-rRNA, but had no effect on the POLR3 transcribed 5S rRNA levels. To our knowledge, this is the first report of flavivirus-induced decrease of specifically POLR1 rRNA transcripts accompanied by host translational shut-off.
Permanent Link: http://hdl.handle.net/11104/0305170