Causes and Effects of Loss of Classical Nonhomologous End Joining Pathway in Parasitic Eukaryotes

0520373 - BC 2020 RIV US eng J - Journal Article
Nenarokova, Anna - Záhonová, Kristýna - Krasilnikova, M. - Gahura, Ondřej - McCulloch, R. - Zíková, Alena - Yurchenko, V. - Lukeš, Julius
Causes and Effects of Loss of Classical Nonhomologous End Joining Pathway in Parasitic Eukaryotes.
mBio. Roč. 10, č. 4 (2019), č. článku e01541-19. ISSN 2161-2129. E-ISSN 2150-7511
R&D Projects: GA ČR(CZ) GA18-15962S; GA ČR(CZ) GA18-17529S; GA MŠMT(CZ) EF16_019/0000759; GA MŠMT(CZ) LL1601
Institutional support: RVO:60077344
Keywords : double-strand breaks * dna-repair * genome sequence * homologous recombination * telomere length * cell-cycle * ku * expression * genes * mechanism * DNA repair * genome size * parasite
OECD category: Microbiology
Impact factor: 6.784, year: 2019
Method of publishing: Open access
https://mbio.asm.org/content/mbio/10/4/e01541-19.full.pdf

We report frequent losses of components of the classical nonhomologous end joining pathway (C-NHEJ), one of the main eukaryotic tools for end joining repair of DNA double-strand breaks, in several lineages of parasitic protists. Moreover, we have identified a single lineage among trypanosomatid flagellates that has lost Ku70 and Ku80, the core C-NHEJ components, and accumulated numerous insertions in many protein-coding genes. We propose a correlation between these two phenomena and discuss the possible impact of the C-NHEJ loss on genome evolution and transition to the parasitic lifestyle.
Permanent Link: http://hdl.handle.net/11104/0305050