Morphology, Ultrastructure, and Mitochondrial Genome of the Marine Non-Photosynthetic Bicosoecid Cafileria marina Gen. et sp. nov.

0519680 - BC 2020 RIV CH eng J - Journal Article
Jirsová, Dagmar - Füssy, Zoltán - Richtová, J. - Gruber, Ansgar - Oborník, Miroslav
Morphology, Ultrastructure, and Mitochondrial Genome of the Marine Non-Photosynthetic Bicosoecid Cafileria marina Gen. et sp. nov.
Microorganisms. Roč. 7, č. 8 (2019), č. článku 240. E-ISSN 2076-2607
R&D Projects: GA ČR(CZ) GA18-13458S; GA MŠMT(CZ) EF16_019/0000759
Institutional support: RVO:60077344
Keywords : messenger-rna * trypanosoma-brucei * sequence * patterson * plastids * model * stramenopiles * communication * organization * chrysophytes * heterotrophic nano-flagellate * heterokonta * bicosoecida * phylogeny * flagellar apparatus * new genus
OECD category: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Impact factor: 4.152, year: 2019
Method of publishing: Open access
https://www.mdpi.com/2076-2607/7/8/240

In this paper, we describe a novel bacteriophagous biflagellate, Cafileria marina with two smooth flagellae, isolated from material collected from a rock surface in the Kvernesfjorden (Norway). This flagellate was characterized by scanning and transmission electron microscopy, fluorescence, and light microscopy. The sequence of the small subunit ribosomal RNA gene (18S) was used as a molecular marker for determining the phylogenetic position of this organism. Apart from the nuclear ribosomal gene, the whole mitochondrial genome was sequenced, assembled, and annotated. Morphological observations show that the newly described flagellate shares key ultrastructural characters with representatives of the family Bicosoecida (Heterokonta). Intriguingly, mitochondria of C. marina frequently associate with its nucleus through an electron-dense disc at the boundary of the two compartments. The function of this association remains unclear. Phylogenetic analyses corroborate the morphological data and place C. marina with other sequence data of representatives from the family Bicosoecida. We describe C. marina as a new species from a new genus in this family.
Permanent Link: http://hdl.handle.net/11104/0304676