Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae)

0519676 - BC 2020 RIV GB eng J - Journal Article
Horčičková, Michaela - Čondlová, Šárka - Holubová, Nikola - Sak, Bohumil - Květoňová, Dana - Hlásková, Lenka - Konečný, R. - Sedláček, F. - Clark, M. - Giddings, C. - McEvoy, J. - Kváč, Martin
Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae).
Parasitology. Roč. 146, č. 2 (2019), s. 220-233. ISSN 0031-1820. E-ISSN 1469-8161
R&D Projects: GA MŠMT(CZ) LTAUSA17165; GA MŠMT(CZ) LM2015062
Institutional support: RVO:60077344
Keywords : boars sus-scrofa * molecular characterization * clethrionomys-glareolus * giardia spp. * wild rodents * c-muris * parvum * prevalence * genotypes * suis * Experimental infection * molecular analyses * oocyst size * phylogeny * Rodentia * voles
OECD category: Veterinary science
Impact factor: 2.783, year: 2019
Method of publishing: Open access
https://www.cambridge.org/core/journals/parasitology/article/diversity-of-cryptosporidium-in-common-voles-and-description-of-cryptosporidium-alticolis-sp-n-and-cryptosporidium-microti-sp-n-apicomplexa-cryptosporidiidae/9667CA24CCAE50F331A284F0C58F861F

Fecal samples from wild-caught common voles (n = 328) from 16 locations in the Czech Republic were screened for Cryptosporidium by microscopy and PCR/sequencing at loci coding small-subunit rRNA, Cryptosporidium oocyst wall protein, actin and 70 kDa heat shock protein. Cryptosporidium infections were detected in 74 voles (22.6%). Rates of infection did not differ between males and females nor between juveniles and adults. Phylogenetic analysis revealed the presence of eight Cryptosporidium species/genotypes including two new species, C. alticolis and C. microti. These species from wild-caught common voles were able to infect common and meadow voles under experimental conditions, with a prepatent period of 3-5 days post-infection (DPI), but they were not infectious for various other rodents or chickens. Meadow voles lost infection earlier than common voles (11-14 vs 13-16 DPI) and had significantly lower infection intensity. Cryptosporidium alticolis infects the anterior small intestine and has larger oocysts (5.4 x 4.9 mu m), whereas C. microti infects the large intestine and has smaller oocysts (4.3 x 4.1 mu m). None of the rodents developed clinical signs of infection. Genetic and biological data support the establishment of C. alticolis and C. microti as separate species of the genus Cryptosporidium.
Permanent Link: http://hdl.handle.net/11104/0304672