Pigment-protein complexes are organized into stable microdomains in cyanobacterial thylakoids

0519560 - MBÚ 2020 RIV NL eng J - Journal Article
Strašková, Adéla - Steinbach, Gabor - Konert, Grzegorz - Kotabová, Eva - Komenda, Josef - Tichý, Martin - Kaňa, Radek
Pigment-protein complexes are organized into stable microdomains in cyanobacterial thylakoids.
Biochimica Et Biophysica Acta-Bioenergetics. Roč. 1860, č. 12 (2019), č. článku 148053. ISSN 0005-2728. E-ISSN 1879-2650
R&D Projects: GA ČR(CZ) GA16-10088S; GA MŠMT(CZ) LO1416; GA MŠMT(CZ) ED2.1.00/19.0392
Institutional support: RVO:61388971
Keywords : Photosynthesis * Confocal microscopy * Thylakoid membrane
OECD category: Microbiology
Impact factor: 3.465, year: 2019
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0005272819300908?via%3Dihub

Thylakoids are the place of the light-photosynthetic reactions. To gain maximal efficiency, these reactions are conditional to proper pigment-pigment and protein-protein interactions. In higher plants thylakoids, the interactions lead to a lateral asymmetry in localization of protein complexes (i.e. granal/stromal thylakoids) that have been defined as a domain-like structures characteristic by different biochemical composition and function (Albertsson P-A. 2001,Trends Plant Science 6: 349-354). We explored this complex organization of thylakoid pigment-proteins at single cell level in the cyanobacterium Synechocysas sp. PCC 6803. Our 3D confocal images captured heterogeneous distribution of all main photosynthetic pigment-protein complexes (PPCs), Photosystem I (fluorescently tagged by YFP), Photosystem II and Phycobilisomes. The acquired images depicted cyanobacterial thylakoid membrane as a stable, mosaic-like structure formed by microdomains (MDs). These microcompartments are of sub-micrometer in sizes (similar to 0.5-1.5 mu m), typical by particular PPCs ratios and importantly without full segregation of observed complexes. The most prevailing MD is represented by MD with high Photosystem I content which allows also partial separation of Photosystems like in higher plants thylakoids. We assume that MDs stability (in minutes) provides optimal conditions for efficient excitation/electron transfer. The cyanobacterial MDs thus define thylakoid membrane organization as a system controlled by co-localization of three main PPCs leading to formation of thylakoid membrane mosaic. This organization might represent evolutional and functional precursor for the granal/stromal spatial heterogeneity in photosystems that is typical for higher plant thylakoids.
Permanent Link: http://hdl.handle.net/11104/0304557