Morphology and secondary chemistry in species recognition of Parmelia omphalodes group evidence from molecular data with notes on the ecological niche modelling and genetic variability of photobionts

0519230 - ÚVGZ 2020 RIV BG eng J - Journal Article
Ossowska, E. - Guzow-Krzemińska, B. - Kolanowska, Marta - Szczepańska, K. - Kukwa, M.
Morphology and secondary chemistry in species recognition of Parmelia omphalodes group evidence from molecular data with notes on the ecological niche modelling and genetic variability of photobionts.
MycoKeys. Roč. 61, DEC 2019 (2019), s. 39-74. ISSN 1314-4057. E-ISSN 1314-4049
Institutional support: RVO:86652079
Keywords : vegetatively reproducing lichen * multiple sequence alignment * family parmeliaceae * adjacent territories * parmotrema-tinctorum * algal symbionts * forming fungi * p. serrana * diversity * rdna * Ascomycota * Parmeliaceae * parmelioid lichens * ITS rDNA * secondary metabolites * morphology * photobiont * ecological niche modelling
OECD category: Plant sciences, botany
Impact factor: 2.525, year: 2019
Method of publishing: Open access
https://mycokeys.pensoft.net/article/38175/

To evaluate the importance of morphological and chemical characters used in the recognition of species within the Parimelia omphalodes group, we performed phylogenetic, morphological and chemical analyses of 335 specimens, of which 34 were used for molecular analyses. Phylogenetic analyses, based on ITS rDNA sequences, show that P pinnatifida is distinct from P omphalodes and the most important difference between those species is the development of pseudocyphellae. In P pinnatifida, they are mostly marginal and form white rims along lobes margins, but laminal pseudocyphellae can develop in older parts of thalli and are predominantly connected with marginal pseudocyphellae. In contrast, in P. omphalodes laminal pseudocyphellae are common and arc predominantly not connected to marginal pseudocyphellae. Chemical composition of secondary lichen metabolites in both analysed species is identical and therefore this feature is not diagnostic in species recognition. Few samples of P. discordans, species morphologically similar to P. omphalodes and P. pinnatifida, were also included in the analyses and they are nested within the clade of P. omphalodes, despite the different chemistry (protocetraric acid present versus salazinic acid in P. omphalodes). All taxa of the P. omphalodes group occupy similar niches, but their potential distributions are wider than those currently known. The absence of specimens in some localities may be limited by the photobiont availability. Parmelia omphalodes and P. pinnatifida are moderately selective in photobiont choice as they form associations with at least two or three lineages of Trebouxia clade S. Parmelia pinnatifida, as well as P. discordans are associated with Trebouxia OTU S02 which seems to have a broad ecological amplitude. Other lineages of Trebouxia seem to be rarer, especially Trebouxia sp. OTU S04, which is sometimes present in P. pinnatifida. This study indicates the importance of extensive research including morphology, chemistry and analysis of molecular markers of both bionts in taxonomical studies of lichens.
Permanent Link: http://hdl.handle.net/11104/0304248