0519214 - BFÚ 2020 NL eng J - Journal Article
Hloušková, P. - Černý, M. - Korinkova, N. - Luklová, M. - Gomez Minguet, E. - Brzobohatý, Břetislav - Galuszka, P. - Bergougnoux, V.
Affinity chromatography revealed 14-3-3 interactome of tomato (Solanum lycopersicum L.) during blue light-induced de-etiolation.
Journal of Proteomics. Roč. 193, FEB 20 2019 (2019), s. 44-61. ISSN 1874-3919. E-ISSN 1876-7737
Institutional support: RVO:68081707
Keywords : membrane h+-atpase * gene-expression data * phosphoenolpyruvate carboxykinase * v-atpase * hypocotyl elongation
OECD category: Biochemistry and molecular biology
Impact factor: 3.509, year: 2019
Method of publishing: Open access
https://doi.org/10.1016/j.jprot.2018.12.017

De-etiolation is the first developmental process under light control allowing the heterotrophic seedling to become autotrophic. The phytohormones cytokinins (CKs) largely contribute to this process. Reversible phosphorylation is a key event of cell signaling, allowing proteins to become active or generating a binding site for specific protein interaction. 14-3-3 proteins regulate a variety of plant responses. The expression, hormonal regulation, and proteomic network under the control of 14-3-3s were addressed in tomato (Solanum lycopersicum L.) during blue light-induced photomorphogenesis. Two isoforms were specifically investigated due to their high expression during tomato de-etiolation. The multidisciplinary approach demonstrated that TFT9 expression, but not TFT6, was regulated by CKs and identified cis-regulating elements required for this response. Our study revealed > 130 potential TFT6/9 interactors. Their functional annotation predicted that TFTs might regulate the activity of proteins involved notably in cell wall strengthening or primary metabolism. Several potential interactors were also predicted to be CK-responsive. For the first time, the 14-3-3 interactome linked to de-etiolation was investigated and evidenced that 14-3-3s might be involved in CK signaling pathway, cell expansion inhibition and steady-state growth rate establishment, and reprograming from heterotrophy to autotrophy.
Permanent Link: http://hdl.handle.net/11104/0304231