Cyclic changes in the amide bands within Escherichia coli biofilms monitored using real-time infrared attenuated total reflection spectroscopy (IR-ATR)

0519146 - FZÚ 2020 RIV US eng J - Journal Article
Štenclová, Pavla - Freisinger, S. - Barth, H. - Kromka, Alexander - Mizaikoff, B.
Cyclic changes in the amide bands within Escherichia coli biofilms monitored using real-time infrared attenuated total reflection spectroscopy (IR-ATR).
Applied Spectroscopy. Roč. 73, č. 4 (2019), s. 424-432. ISSN 0003-7028. E-ISSN 1943-3530
Grant - others:AV ČR(CZ) MSM100101703
Program: Program na podporu mezinárodní spolupráce začínajících výzkumných pracovníků
Institutional support: RVO:68378271
Keywords : Escherichia coli * bacteria * infrared attenuated total reflectance * IR-ATR spectroscopy
OECD category: Biophysics
Impact factor: 2.087, year: 2019
Method of publishing: Limited access
https://doi.org/10.1177/0003702819829081

Contrary to the planktonic state of bacteria, their biofilm form represents severe complications in areas such as human medicine or food industry due to the increasing resistance against harsh conditions and treatment. In the present study, infrared attenuated total reflection (IR-ATR) spectroscopy has been applied as an analytic tool studying Escherichia coli (E. coli) biofilm formation close to real time. We report on IR spectroscopic investigations on the biofilm formation via ATR waveguides probing the biofilm in the spectral window of 1800–900 cm−1 at dynamic flow conditions, which facilitated monitoring the growth dynamics during several days. Observing this behavior via IR spectroscopy allows revealing these cyclical changes in bottom-layered bacteria within the biofilm under continuous nutrient flow, in molecular detail, and during extended periods for the first time.
Permanent Link: http://hdl.handle.net/11104/0304168