ISWI ATPase Smarca5 Regulates Differentiation of Thymocytes Undergoing beta-Selection

0518837 - ÚEM 2020 RIV US eng J - Journal Article
Zikmund, T. - Kokavec, J. - Turková, T. - Savvulidi, F. - Paszeková, H. - Vodenková, Soňa - Sedláček, Radislav - Skoultchi, A. - Stopka, T.
ISWI ATPase Smarca5 Regulates Differentiation of Thymocytes Undergoing beta-Selection.
Journal of Immunology. Roč. 202, č. 12 (2019), s. 3434-3446. ISSN 0022-1767. E-ISSN 1550-6606
R&D Projects: GA MŠMT(CZ) LM2015040; GA MŠMT(CZ) LQ1604; GA MŠMT ED2.1.00/19.0395; GA MŠMT(CZ) ED1.1.00/02.0109; GA ČR(CZ) GA19-10543S; GA MZd(CZ) NV17-30920A; GA MŠMT ED2.1.00/19.0395
Institutional support: RVO:68378041 ; RVO:68378050
Keywords : chromatin-remodeling complexes * DNA-damage * lymphocyte development
OECD category: Human genetics; Human genetics (UMG-J)
Impact factor: 4.886, year: 2019
Method of publishing: Open access with time embargo
https://www.jimmunol.org/content/202/12/3434

Development of lymphoid progenitors requires a coordinated regulation of gene expression, DNA replication, and gene rearrangement. Chromatin-remodeling activities directed by SWI/SNF2 superfamily complexes play important roles in these processes. In this study, we used a conditional knockout mouse model to investigate the role of Smarca5, a member of the ISWI subfamily of such complexes, in early lymphocyte development. Smarca5 deficiency results in a developmental block at the DN3 stage of alpha beta thymocytes and pro-B stage of early B cells at which the rearrangement of Ag receptor loci occurs. It also disturbs the development of committed (CD73(+)) gamma delta thymocytes. The alpha beta thymocyte block is accompanied by massive apoptotic depletion of beta-selected double-negative DN3 cells and premitotic arrest of CD4/CD8 double-positive cells. Although Smarca5-deficient alpha beta T cell precursors that survived apoptosis were able to undergo a successful TCR beta rearrangement, they exhibited a highly abnormal mRNA profile, including the persistent expression of CD44 and CD25 markers characteristic of immature cells. We also observed that the p53 pathway became activated in these cells and that a deficiency of p53 partially rescued the defect in thymus cellularity (in contrast to early B cells) of Smarca5-deficient mice. However, the activation of p53 was not primarily responsible for the thymocyte developmental defects observed in the Smarca5 mutants. Our results indicate that Smarca5 plays a key role in the development of thymocytes undergoing beta-selection, gamma delta thymocytes, and also B cell progenitors by regulating the transcription of early differentiation programs.
Permanent Link: http://hdl.handle.net/11104/0303868