Development of breeding methods for increased lipids accumulation by marine microalgae using fluorescence-activated cell sorting

0518558 - ÚVGZ 2020 FR eng A - Abstract
Búzová, Diana - Literáková, Petra - Fedorko, Jan - Kaštánek, P. - Červený, Jan
Development of breeding methods for increased lipids accumulation by marine microalgae using fluorescence-activated cell sorting.
Book of abstract: 9th European Symposium on Plant Lipids. Marseille, 2019 - (Beisson, F.; Li-Beisson, Y.). s. 52-53
[9th European Symposium on Plant Lipids. 07.07.2019-10.07.2019, Marseille]
R&D Projects: GA TA ČR(CZ) TN01000048; GA MŠMT(CZ) LO1415; GA MŠMT(CZ) LM2015055
Institutional support: RVO:86652079
Keywords : breeding methods * lipids accumulation * fluorescence-activated cell sorting * microalgae

Development of breeding methods for increased lipids accumulation by marine microalgae using fluorescence-activated cell sorting. The marine microorganism belonging to the family Thraustochytriidae are group of non-photosynthetic marine protists. These unicellular, heterotrophic eukaryotes are commonly found in seawater and sediments, with the highest abundance in nutrient-rich areas, such as mangrove forests. Thraustochytrids are reported to be efficient producers of polyunsaturated fatty acids (PUFA) including the n-3 series, such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). These organisms are considered as one of the potential alternative sources of PUFA for commercial and industrial exploitation. We developed advanced cultivation strategy and breeding method, and applied them on one particular Thraustochytrid strain Schizochytrium AN4 for optimization of lipids accumulation. The breeding method aims for selection of the hyper producing subpopulation using high-throughput fluorescence-activated cell sorting (FACS) in combination with imaging flow cytometry (IFC) and multiparametric discrimination the cells with both high lipid content and metabolic activity. The method was tested for negative influence on post-sorting cell viability possibly caused by the sorting technique procedure (e.g. high pressure, laser beam exposure, electro-magnetic field, toxic effects of both fluorescent marker and fluidic system medium composition) with no effect compared to non-sorted control sample.
Permanent Link: http://hdl.handle.net/11104/0303670