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Chromosome Painting Facilitates Anchoring Reference Genome Sequence to Chromosomes In Situ and Integrated Karyotyping in Banana (Musa Spp.)

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    0518420 - ÚEB 2020 RIV CH eng J - Journal Article
    Šimoníková, Denisa - Němečková, Alžběta - Karafiátová, Miroslava - Uwimana, B. - Swennen, R. - Doležel, Jaroslav - Hřibová, Eva
    Chromosome Painting Facilitates Anchoring Reference Genome Sequence to Chromosomes In Situ and Integrated Karyotyping in Banana (Musa Spp.).
    Frontiers in Plant Science. Roč. 10, NOV 20 (2019), č. článku 1503. ISSN 1664-462X. E-ISSN 1664-462X
    R&D Projects: GA ČR(CZ) GA19-20303S
    Institutional support: RVO:61389030
    Keywords : repetitive dna-sequences * flow-cytometric analysis * ribosomal-rna genes * 5s rdna * fish * hybridization * wheat * identification * localization * acuminata * banana * chromosome identification * fluorescence in situ hybridization * molecular karyotype * Musa * oligo painting FISH
    OECD category: Agricultural biotechnology and food biotechnology
    Impact factor: 4.402, year: 2019
    Method of publishing: Open access
    http://dx.doi.org/10.3389/fpls.2019.01503

    Oligo painting FISH was established to identify all chromosomes in banana (Musa spp.) and to anchor pseudomolecules of reference genome sequence of Musa acuminata spp. malaccensis DH Pahang to individual chromosomes in situ. A total of 19 chromosome/chromosome-arm specific oligo painting probes were developed and were shown to be suitable for molecular cytogenetic studies in genus Musa. For the first time, molecular karyotypes of diploid M. acuminata spp. malaccensis (A genome), M. balbisiana (B genome), and M. schizocarpa (S genome) from the Eumusa section of Musa, which contributed to the evolution of edible banana cultivars, were established. This was achieved after a combined use of oligo painting probes and a set of previously developed banana cytogenetic markers. The density of oligo painting probes was sufficient to study chromosomal rearrangements on mitotic as well as on meiotic pachytene chromosomes. This advance will enable comparative FISH mapping and identification of chromosomal translocations which accompanied genome evolution and speciation in the family Musaceae.
    Permanent Link: http://hdl.handle.net/11104/0303668

     
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